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A polymerase chain reaction (PCR) assay was developed for the detection of alcelaphine herpesvirus 1 (AHV1), a causative agent of malignant catarrhal fever (MCF) of ruminants. A pair of 20-base primers was constructed based on the published nucleotide sequence of gene A of the WC11 isolate of AHV1 and was used to amplify a DNA fragment of 413 base pairs. The optimised PCR assay was highly sensitive, i.e. it detected 10 fg of genomic DNA of AHV1 (WC11 isolate). The amplified fragment was shown to be specific for AHV1 DNA by (i) cleavage with XbaI which yielded 2 subfragments of approximately 140 and 280 base pairs and (ii) chemiluminescence Southern blot hybridisation with a digoxigenin-labelled 25-base internal probe. The PCR assay also amplified AHV1 gene sequences in tissue samples from deer and rabbits experimentally infected with materials derived from deer with clinical sheep-associated MCF.
K M Tham, and
K Ng, and
L W Young
January 1983,
Journal of comparative pathology,
K M Tham, and
K Ng, and
L W Young
March 1999,
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
K M Tham, and
K Ng, and
L W Young
January 1984,
Comparative immunology, microbiology and infectious diseases,
K M Tham, and
K Ng, and
L W Young
July 1994,
Journal of wildlife diseases,
K M Tham, and
K Ng, and
L W Young
November 1999,
The Veterinary record,
K M Tham, and
K Ng, and
L W Young
March 1988,
The Onderstepoort journal of veterinary research,
K M Tham, and
K Ng, and
L W Young
November 2007,
The Veterinary record,
K M Tham, and
K Ng, and
L W Young
April 1999,
The Veterinary record,
K M Tham, and
K Ng, and
L W Young
December 2018,
Transboundary and emerging diseases,
K M Tham, and
K Ng, and
L W Young
January 1982,
Comparative immunology, microbiology and infectious diseases,
