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Bull seminal plasma contains high levels of beta-glucuronidase. The present study describes the isolation and characterization of beta-glucuronidase, and its role in fertilization. beta-glucuronidase was purified by ion exchange chromatography, saccharolactone-agarose affinity chromatography, and gel filtration. The specific activity of the purified enzyme was 4,414 mumoles/mg protein/min. The purified enzyme showed a single band on 7.5% PAGE. On SDS-PAGE, the enzyme appeared to consist of four identical subunits of M(r) 75,000 each. The apparent Km and Vmax for beta-glucuronidase were 0.4 mM and 5.7 mumol/min using phenolpthalein mono-beta-glucuronic acid as the substrate. beta-glucuronidase appeared to accelerate the cumulus dispersion in vitro.
A Rethinaswamy, and
C H Yang, and
P N Srivastava
January 1986,
Enzyme,
A Rethinaswamy, and
C H Yang, and
P N Srivastava
June 1995,
The international journal of biochemistry & cell biology,
A Rethinaswamy, and
C H Yang, and
P N Srivastava
February 1978,
Acta endocrinologica,
A Rethinaswamy, and
C H Yang, and
P N Srivastava
November 1983,
Biochimica et biophysica acta,
A Rethinaswamy, and
C H Yang, and
P N Srivastava
June 1986,
Biochemical and biophysical research communications,
A Rethinaswamy, and
C H Yang, and
P N Srivastava
December 1992,
Biochemistry international,
A Rethinaswamy, and
C H Yang, and
P N Srivastava
July 1977,
Biochimica et biophysica acta,
A Rethinaswamy, and
C H Yang, and
P N Srivastava
March 1976,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
A Rethinaswamy, and
C H Yang, and
P N Srivastava
January 1983,
American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction,
A Rethinaswamy, and
C H Yang, and
P N Srivastava
January 1983,
Microbiology and immunology,
