BIOMAT Database Logo BIOMAT Database Logo

Probing protein-protein interactions. The ribose-binding protein in bacterial transport and chemotaxis. 1994

A J Björkman, and R A Binnie, and H Zhang, and L B Cole, and M A Hermodson, and S L Mowbray
Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala.

A number of mutations at Gly134 of the periplasmic ribose-binding protein of Escherichia coli were examined by a combined biochemical and structural approach. Different mutations gave rise to different patterns of effects on the chemotaxis and transport functions. The smallest residue (alanine) had the least effect on transport, whereas large hydrophobic residues had the smallest effect on chemotaxis. Comparison of the x-ray crystal structure of the G134R mutant protein (2.5-A resolution) to that of the wild type (1.6-A resolution) showed that the basic structure of the protein was unaltered. The loss of chemotaxis and transport functions in this and similar mutant proteins must therefore be caused by relatively simple surface effects, which include a change in local main chain conformation. The loss of chemotaxis and transport functions resulting from the introduction of an alanine residue at position 134 was suppressed by an additional isoleucine to threonine mutation at residue 132. An x-ray structure of the I132T/G134A double mutant protein (2.2-A resolution) showed that the changes in local structure were accompanied by a diffuse pattern of structural changes in the surrounding region, implying that the suppression derives from a combination of sources.

UI MeSH Term Description Entries
D008958 Models, Molecular Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures. Molecular Models,Model, Molecular,Molecular Model
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D002352 Carrier Proteins Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes. Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D002633 Chemotaxis The movement of cells or organisms toward or away from a substance in response to its concentration gradient. Haptotaxis
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005998 Glycine A non-essential amino acid. It is found primarily in gelatin and silk fibroin and used therapeutically as a nutrient. It is also a fast inhibitory neurotransmitter. Aminoacetic Acid,Glycine, Monopotassium Salt,Glycine Carbonate (1:1), Monosodium Salt,Glycine Carbonate (2:1), Monolithium Salt,Glycine Carbonate (2:1), Monopotassium Salt,Glycine Carbonate (2:1), Monosodium Salt,Glycine Hydrochloride,Glycine Hydrochloride (2:1),Glycine Phosphate,Glycine Phosphate (1:1),Glycine Sulfate (3:1),Glycine, Calcium Salt,Glycine, Calcium Salt (2:1),Glycine, Cobalt Salt,Glycine, Copper Salt,Glycine, Monoammonium Salt,Glycine, Monosodium Salt,Glycine, Sodium Hydrogen Carbonate,Acid, Aminoacetic,Calcium Salt Glycine,Cobalt Salt Glycine,Copper Salt Glycine,Hydrochloride, Glycine,Monoammonium Salt Glycine,Monopotassium Salt Glycine,Monosodium Salt Glycine,Phosphate, Glycine,Salt Glycine, Monoammonium,Salt Glycine, Monopotassium,Salt Glycine, Monosodium
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D012266 Ribose A pentose active in biological systems usually in its D-form. D-Ribose,D Ribose
D016297 Mutagenesis, Site-Directed Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion. Mutagenesis, Oligonucleotide-Directed,Mutagenesis, Site-Specific,Oligonucleotide-Directed Mutagenesis,Site-Directed Mutagenesis,Site-Specific Mutagenesis,Mutageneses, Oligonucleotide-Directed,Mutageneses, Site-Directed,Mutageneses, Site-Specific,Mutagenesis, Oligonucleotide Directed,Mutagenesis, Site Directed,Mutagenesis, Site Specific,Oligonucleotide Directed Mutagenesis,Oligonucleotide-Directed Mutageneses,Site Directed Mutagenesis,Site Specific Mutagenesis,Site-Directed Mutageneses,Site-Specific Mutageneses

Related Publications

A J Björkman, and R A Binnie, and H Zhang, and L B Cole, and M A Hermodson, and S L Mowbray
The role of ribose-binding protein in transport and chemotaxis in Escherichia coli K12.
December 1977, Archives of biochemistry and biophysics,
A J Björkman, and R A Binnie, and H Zhang, and L B Cole, and M A Hermodson, and S L Mowbray
A novel calcium binding site in the galactose-binding protein of bacterial transport and chemotaxis.
January 1987, Nature,
A J Björkman, and R A Binnie, and H Zhang, and L B Cole, and M A Hermodson, and S L Mowbray
Conformational changes of three periplasmic receptors for bacterial chemotaxis and transport: the maltose-, glucose/galactose- and ribose-binding proteins.
November 1996, Journal of molecular biology,
A J Björkman, and R A Binnie, and H Zhang, and L B Cole, and M A Hermodson, and S L Mowbray
Functional mapping of the surface of Escherichia coli ribose-binding protein: mutations that affect chemotaxis and transport.
December 1992, Protein science : a publication of the Protein Society,
A J Björkman, and R A Binnie, and H Zhang, and L B Cole, and M A Hermodson, and S L Mowbray
Identification of the ribose binding protein as the receptor for ribose chemotaxis in Salmonella typhimurium.
October 1974, Biochemistry,
A J Björkman, and R A Binnie, and H Zhang, and L B Cole, and M A Hermodson, and S L Mowbray
Molecular interactions in ribose transport: the binding protein module symmetrically associates with the homodimeric membrane transporter.
August 1999, The EMBO journal,
A J Björkman, and R A Binnie, and H Zhang, and L B Cole, and M A Hermodson, and S L Mowbray
Ribose and glucose-galactose receptors. Competitors in bacterial chemotaxis.
September 1992, Journal of molecular biology,
A J Björkman, and R A Binnie, and H Zhang, and L B Cole, and M A Hermodson, and S L Mowbray
Genetically probing the regions of ribose-binding protein involved in permease interaction.
August 1996, Molecular microbiology,
A J Björkman, and R A Binnie, and H Zhang, and L B Cole, and M A Hermodson, and S L Mowbray
Rates of ligand binding to periplasmic proteins involved in bacterial transport and chemotaxis.
November 1983, The Journal of biological chemistry,
A J Björkman, and R A Binnie, and H Zhang, and L B Cole, and M A Hermodson, and S L Mowbray
Probing the relation between protein-protein interactions and DNA binding for a linker mutant of the bacterial nucleoid protein H-NS.
February 2014, Biochimica et biophysica acta,
Copied contents to your clipboard!