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Five different target regions along the length of the dengue virus type 2 genome were compared for inhibition of the virus following intracellular injection of the cognate antisense oligonucleotides and their analogs. Unmodified phosphodiester oligonucleotides as well as the corresponding phosphorothioate oligonucleotides were ineffective in bringing about a significant inhibition of the virus. Novel modified phosphorothioate oligonucleotides in which the C-5 atoms of uridines and cytidines were replaced by propynyl groups caused a significant inhibition of the virus. Antisense oligonucleotide directed against the target region near the translation initiation site of dengue virus RNA was the most effective, followed by antisense oligonucleotide directed against a target in the 3' untranslated region of the virus RNA. It is suggested that the inhibitory effect of these novel modified oligonucleotides is due to their increased affinity for the target sequences and that they probably function via an RNase H cleavage of the oligonucleotide:RNA heteroduplex.
K Raviprakash, and
K Liu, and
M Matteucci, and
R Wagner, and
R Riffenburgh, and
M Carl
January 1991,
Nucleic acids symposium series,
K Raviprakash, and
K Liu, and
M Matteucci, and
R Wagner, and
R Riffenburgh, and
M Carl
April 1999,
Antisense & nucleic acid drug development,
K Raviprakash, and
K Liu, and
M Matteucci, and
R Wagner, and
R Riffenburgh, and
M Carl
February 1996,
Acta virologica,
K Raviprakash, and
K Liu, and
M Matteucci, and
R Wagner, and
R Riffenburgh, and
M Carl
January 1993,
Nucleic acids symposium series,
K Raviprakash, and
K Liu, and
M Matteucci, and
R Wagner, and
R Riffenburgh, and
M Carl
September 1993,
FEBS letters,
K Raviprakash, and
K Liu, and
M Matteucci, and
R Wagner, and
R Riffenburgh, and
M Carl
April 2024,
RSC advances,
K Raviprakash, and
K Liu, and
M Matteucci, and
R Wagner, and
R Riffenburgh, and
M Carl
January 1996,
Biochemical and biophysical research communications,
K Raviprakash, and
K Liu, and
M Matteucci, and
R Wagner, and
R Riffenburgh, and
M Carl
January 2000,
Molekuliarnaia biologiia,
K Raviprakash, and
K Liu, and
M Matteucci, and
R Wagner, and
R Riffenburgh, and
M Carl
January 2000,
Methods in enzymology,
K Raviprakash, and
K Liu, and
M Matteucci, and
R Wagner, and
R Riffenburgh, and
M Carl
October 1989,
Anti-cancer drug design,
