Biomaterial Database

Comparison of the labeled avidin-biotin and the conventional enzyme-linked immunosorbent assay for detecting antibody to reovirus in chickens. 1994

L H Lee, and Y H Wang, and J H Shien

Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan.

A labeled avidin-biotin enzyme-linked immunosorbent assay (LAB-ELISA) for detecting antibodies to avian reovirus (ARV) in chicken sera was developed and compared with conventional ELISA. Purified ARV, biotin-labeled rabbit anti-chicken IgG conjugate, and horseradish peroxidase-labeled avidin were used in the LAB-ELISA. The two types of ELISA had the similar ability to clearly distinguish ARV-positive and -negative sera. Furthermore, non-specific reactions with the two ELISAs against sera from ARV-negative chickens were reduced markedly. When sera from farm chickens were tested by the two ELISAs and serum neutralization (SN), the correlation rates between SN and conventional ELISA, and SN and LAB-ELISA were similar and were 93.4% and 92.3%, respectively.

UI	MeSH Term	Description	Entries
D009500	Neutralization Tests	The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).	Neutralization Test,Test, Neutralization,Tests, Neutralization
D011201	Poultry Diseases	Diseases of birds which are raised as a source of meat or eggs for human consumption and are usually found in barnyards, hatcheries, etc. The concept is differentiated from BIRD DISEASES which is for diseases of birds not considered poultry and usually found in zoos, parks, and the wild.	Disease, Poultry,Diseases, Poultry,Poultry Disease
D012087	Reoviridae	A family of unenveloped RNA viruses with cubic symmetry. The twelve genera include ORTHOREOVIRUS; ORBIVIRUS; COLTIVIRUS; ROTAVIRUS; Aquareovirus, Cypovirus, Phytoreovirus, Fijivirus, Seadornavirus, Idnoreovirus, Mycoreovirus, and Oryzavirus.	Aquareovirus,Cypovirus,Cytoplasmic Polyhedrosis Viruses,Fijivirus,Idnoreovirus,Mycoreovirus,Oryzavirus,Phytoreovirus,Reoviruses, Aquatic,Respiratory Enteric Orphan Viruses,Seadornavirus,Aquareoviruses,Aquatic Reovirus,Aquatic Reoviruses,Cypoviruses,Cytoplasmic Polyhedrosis Virus,Fijiviruses,Idnoreoviruses,Mycoreoviruses,Oryzaviruses,Phytoreoviruses,Polyhedrosis Virus, Cytoplasmic,Polyhedrosis Viruses, Cytoplasmic,Reovirus, Aquatic,Seadornaviruses
D012088	Reoviridae Infections	Infections produced by reoviruses, general or unspecified.	Reovirus Infections,Infections, Reoviridae,Infection, Reoviridae,Infection, Reovirus,Infections, Reovirus,Reoviridae Infection,Reovirus Infection
D002645	Chickens	Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.	Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D004797	Enzyme-Linked Immunosorbent Assay	An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.	ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005069	Evaluation Studies as Topic	Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies.	Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000914	Antibodies, Viral	Immunoglobulins produced in response to VIRAL ANTIGENS.	Viral Antibodies
D001360	Avidin	A specific protein in egg albumin that interacts with BIOTIN to render it unavailable to mammals, thereby producing biotin deficiency.