-transparent.png){kind=logo}
A recombinant plasmid providing for the synthesis and secretion of the human atrial natriuretic peptide (hANP) as a C-terminal hybrid with the St. aureus protein A was constructed. The level of secretion of the chimeric proteins and their proteolytic stability were shown to depend upon the genotype of the recipient strains and the cultivation conditions. The hybrid proteins were purified by chromatography on IgG Sepharose. The presence of peptides corresponding to the hANP in the acid hydrolysates of the secreted and affinity-purified proteins was confirmed by the enzyme-linked immunoassay and analytical HPLC.
Related Publications
M N Nikolaidis, and
A Sh Parsadanian, and
G E Pozmogova, and
M A El'darov, and
N M Anan'eva, and
N A Maĭsurian, and
A A Galoian
November 1985,
Proceedings of the National Academy of Sciences of the United States of America,
M N Nikolaidis, and
A Sh Parsadanian, and
G E Pozmogova, and
M A El'darov, and
N M Anan'eva, and
N A Maĭsurian, and
A A Galoian
December 1991,
Science in China. Series B, Chemistry, life sciences & earth sciences,
M N Nikolaidis, and
A Sh Parsadanian, and
G E Pozmogova, and
M A El'darov, and
N M Anan'eva, and
N A Maĭsurian, and
A A Galoian
July 1986,
Journal of the American College of Cardiology,
M N Nikolaidis, and
A Sh Parsadanian, and
G E Pozmogova, and
M A El'darov, and
N M Anan'eva, and
N A Maĭsurian, and
A A Galoian
June 1988,
European journal of biochemistry,
M N Nikolaidis, and
A Sh Parsadanian, and
G E Pozmogova, and
M A El'darov, and
N M Anan'eva, and
N A Maĭsurian, and
A A Galoian
September 1990,
The American journal of physiology,
M N Nikolaidis, and
A Sh Parsadanian, and
G E Pozmogova, and
M A El'darov, and
N M Anan'eva, and
N A Maĭsurian, and
A A Galoian
November 1992,
Circulation research,
M N Nikolaidis, and
A Sh Parsadanian, and
G E Pozmogova, and
M A El'darov, and
N M Anan'eva, and
N A Maĭsurian, and
A A Galoian
February 1988,
Molecular and cellular endocrinology,
M N Nikolaidis, and
A Sh Parsadanian, and
G E Pozmogova, and
M A El'darov, and
N M Anan'eva, and
N A Maĭsurian, and
A A Galoian
June 1989,
Archives of biochemistry and biophysics,
M N Nikolaidis, and
A Sh Parsadanian, and
G E Pozmogova, and
M A El'darov, and
N M Anan'eva, and
N A Maĭsurian, and
A A Galoian
April 1988,
FEBS letters,
M N Nikolaidis, and
A Sh Parsadanian, and
G E Pozmogova, and
M A El'darov, and
N M Anan'eva, and
N A Maĭsurian, and
A A Galoian
June 1992,
Bioorganicheskaia khimiia,
