Biomaterial Database

Plasmid transfer from Escherichia coli to coryneform bacteria by conjugation. 1994

H Qian, and W Fan, and J Wu, and Z Zheng

Institute of Genetics, Fudan University, Shanghai, China.

The mobilizable shuttle plasmids pXZ911, pBZ51, pBZ52, carrying the Mob site and replication origin of the Corynebacteria plasmid pXZ10145, were constructed. In E. coli S17-1, they can be transferred to coryneform bacteria C. glutamicum ATCC13032, C. glutamicum ATCC21543, C. pekinense B3, C. pekinense AS1.299, C. herculis B43, B. flavum ATCC14067 by conjugation and the transfer frequencies are 9 x 10(-5), 1 x 10(-4), 8.5 x 10(-5), 2.3 x 10(-4), 6 x 10(-4), 2.9 x 10(-5), respectively. This plasmid transfer system, which is convenient and highly effective, should greatly facilitate the basic research and optimization of amino acid genetic engineering in coryneform bacteria and related species.

UI	MeSH Term	Description	Entries
D010957	Plasmids	Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.	Episomes,Episome,Plasmid
D003227	Conjugation, Genetic	A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.	Bacterial Conjugation,Conjugation, Bacterial,Genetic Conjugation
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D000192	Actinomycetales	An order of gram-positive, primarily aerobic BACTERIA that tend to form branching filaments.	Corynebacteriaceae,Coryneform Group
D018014	Gene Transfer Techniques	The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.	Gene Delivery Systems,Gene Transfer Technique,Transgenesis,Delivery System, Gene,Delivery Systems, Gene,Gene Delivery System,Technique, Gene Transfer,Techniques, Gene Transfer,Transfer Technique, Gene,Transfer Techniques, Gene