Biomaterial Database

[An improved method for isolating blood monocytes]. 1993

I K Kondakov, and N G Menzianova, and A B Shitova

The authors have improved the method for the gradient isolation of monocytes from human whole blood: an expensive reagent, percoll, is replaced by verograffin, an x-ray contrast agent. Monocytes were isolated by this method from the blood of 8 normal subjects. The isolated monocytes were identified and their population composition studied electronmicroscopically, cytochemically, and immunocytochemically. The monocyte population of healthy donors was found morphologically and cytochemically heterogeneous, consisting of 4 types of cells, forming on the monocytogram stable (in their per cent shares) classes. The improved method for monocyte isolation and the results of morphometric analysis may be used to assess the system of human mononuclear phagocytes in various diseases.

UI	MeSH Term	Description	Entries
D007150	Immunohistochemistry	Histochemical localization of immunoreactive substances using labeled antibodies as reagents.	Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D007202	Indicators and Reagents	Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)	Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D009000	Monocytes	Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.	Monocyte
D001782	Blood Donors	Individuals supplying blood or blood components for transfer to histocompatible recipients.	Blood Donor,Donor, Blood,Donors, Blood
D002469	Cell Separation	Techniques for separating distinct populations of cells.	Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D003974	Diatrizoate Meglumine	A versatile contrast medium used for DIAGNOSTIC X-RAY RADIOLOGY.	Diatrizoate Methylglucamine,Diatrizoic Acid Methylglucamine,Meglumine Diatrizoate,Methylglucamine Diatrizoate,Amidotricoic Acid,Amidotrizoic Acid,Angiografin,Gastrograffin,Gastrografin,Gastrographin,Ioxeol,Meglumine Amidotrizoate,Reno 60,Reno M-Dip,Renograffin,Renografin,Renografin M-76,Sinografin,Triombrast,Triombrin,Urografin,Urografin 76,Urovist,Verografin,Amidotrizoate, Meglumine,Diatrizoate, Meglumine,Diatrizoate, Methylglucamine,Meglumine, Diatrizoate,Methylglucamine, Diatrizoate,Methylglucamine, Diatrizoic Acid,Reno M Dip,Reno MDip,Renografin M 76,Renografin M76
D006651	Histocytochemistry	Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.	Cytochemistry
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults