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The authors have defined the optimal conditions for inhibitory type enzyme immunoassay of microalbuminuria. The range of albumin detection is 30 to 250 micrograms/ml. The optimal time of antigen binding with antibody is 45 min, the lower threshold level of albumin detection 0.6 +/- 0.02 microgram/ml. Albumin concentration is unchanged after urine storage for 8 weeks at -20 degrees C or for 1 week at 4 degrees C.
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