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[Relationship of histoplasmin intradermal tests and antibodies levels detected by ELISA and immunodiffusion]. 1994

C M Fernandez-Andreu, and A M Cadre-Raton, and G Martinez Machin, and A Llop Iiernandez, and M Suarez Iiernandez
Laboratorio de Micología, Instituto de Medicina Tropical Pedro Kourí (IPK), La Habana, Cuba.

A prospective study was carried out in two groups of individuals: a group 1 (n = 40) included workers from a poultry farm, with potential occupational risk of exposure to Histoplasma capsulatum, etiologic agent of histoplasmosis, and a group 2 (n = 16), persons without occupational risk of exposure to the agent. Histoplasmin skin test was performed in both groups, and three sera were obtained from each individual: 1) before skin test was done, 2) 30 days after, and 3) 180 days after it. In both groups the histoplasmin skin test, even when the test was positive, was not a sufficient antigenic booster to provoke an increase in the H. capsulatum antibody levels capable to be detected by the serologic tests used (ELISA and Double Immunodiffusion). These results contribute to improve the interpretation of ELISA test values in the diagnosis of histoplasmosis.

UI MeSH Term Description Entries
D007428 Intradermal Tests Skin tests in which the sensitizer is injected. Passive Transfer Test,Prausnitz-Kustner Test,Intradermal Test,Passive Transfer Tests,Prausnitz Kustner Test,Test, Intradermal,Test, Passive Transfer,Test, Prausnitz-Kustner,Tests, Intradermal,Tests, Passive Transfer
D011446 Prospective Studies Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group. Prospective Study,Studies, Prospective,Study, Prospective
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005779 Immunodiffusion Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction. Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D006658 Histoplasma A mitosporic Onygenales fungal genus causing HISTOPLASMOSIS in humans and animals. Its single species is Histoplasma capsulatum which has two varieties: H. capsulatum var. capsulatum and H. capsulatum var. duboisii. Its teleomorph is AJELLOMYCES capsulatus. Ajellomyces capsulatus,Cryptococcus capsulatus,Emmonsiella capsulata,Histoplasma capsulatum,Histoplasmas
D006659 Histoplasmin
D006660 Histoplasmosis Infection resulting from exposure to the fungus HISTOPLASMA. African Histoplasmosis,Disseminated Histoplasmosis,Histoplasma duboisii Infection,Pulmonary Histoplasmosis,Histoplasma Infection,Histoplasma capsulatum Infection,Histoplasma Infections,Histoplasma capsulatum Infections,Histoplasma duboisii Infections,Histoplasmosis, African,Histoplasmosis, Disseminated,Histoplasmosis, Pulmonary,Infection, Histoplasma,Infection, Histoplasma capsulatum,Infection, Histoplasma duboisii
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000908 Antibodies, Fungal Immunoglobulins produced in a response to FUNGAL ANTIGENS. Fungal Antibodies

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