BIOMAT Database Logo BIOMAT Database Logo

Identification as beta-adducin of a protein interacting with rabphilin-3A in the presence of Ca2+ and phosphatidylserine. 1994

M Miyazaki, and H Shirataki, and H Kohno, and K Kaibuchi, and A Tsugita, and Y Takai
Department of Molecular Biology and Biochemistry, Osaka University Medical School, Japan.

Rabphilin-3A is a putative target protein for Rab3A small GTP-binding protein implicated in neurotransmitter release. We have previously identified a Rabphilin-3A-interacting protein with a Mr of about 115 kDa in bovine brain. We have attempted here to purify this protein and to determine its primary structure. Amino acid sequence analysis has revealed that this protein is a bovine counterpart of human beta-adducin which is known to be a good substrate for protein kinase C. The Rabphilin-3A-interacting protein also binds to protein kinase C in the presence of Ca2+ and phosphatidylserine. These results indicate that Rabphilin-3A binds to beta-adducin in the presence of Ca2+ and phosphatidylserine.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009419 Nerve Tissue Proteins Proteins, Nerve Tissue,Tissue Proteins, Nerve
D010718 Phosphatidylserines Derivatives of PHOSPHATIDIC ACIDS in which the phosphoric acid is bound in ester linkage to a SERINE moiety. Serine Phosphoglycerides,Phosphatidyl Serine,Phosphatidyl Serines,Phosphatidylserine,Phosphoglycerides, Serine,Serine, Phosphatidyl,Serines, Phosphatidyl
D011487 Protein Conformation The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain). Conformation, Protein,Conformations, Protein,Protein Conformations
D001921 Brain The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM. Encephalon
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002148 Calmodulin-Binding Proteins Proteins which bind calmodulin. They are found in many tissues and have a variety of functions including F-actin cross-linking properties, inhibition of cyclic nucleotide phosphodiesterase and calcium and magnesium ATPases. Caldesmon,Calspectin,CaM-BP(80),Caldesmon (77),Calmodulin Binding Proteins,Proteins, Calmodulin-Binding
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000097624 Rabphilin-3A A putative target protein for RAB3A GTP-BINDING PROTEIN. Implicated in regulated secretion, particularly in neurotransmitter release.

Related Publications

M Miyazaki, and H Shirataki, and H Kohno, and K Kaibuchi, and A Tsugita, and Y Takai
Identification of a rabphilin-3A-interacting protein as GTP cyclohydrolase I in PC12 cells.
December 1994, Biochemical and biophysical research communications,
M Miyazaki, and H Shirataki, and H Kohno, and K Kaibuchi, and A Tsugita, and Y Takai
Phosphorylation of rabphilin-3A by Ca2+/calmodulin- and cAMP-dependent protein kinases in vitro.
March 1995, The Journal of neuroscience : the official journal of the Society for Neuroscience,
M Miyazaki, and H Shirataki, and H Kohno, and K Kaibuchi, and A Tsugita, and Y Takai
Involvement of rabphilin-3A in Ca2+-dependent exocytosis from PC12 cells.
February 1996, Biochemical and biophysical research communications,
M Miyazaki, and H Shirataki, and H Kohno, and K Kaibuchi, and A Tsugita, and Y Takai
Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid-binding protein, depends on rab3A/3C.
October 1994, Neuron,
M Miyazaki, and H Shirataki, and H Kohno, and K Kaibuchi, and A Tsugita, and Y Takai
Rabphilin-3A as a Targeted Autoantigen in Lymphocytic Infundibulo-neurohypophysitis.
July 2015, The Journal of clinical endocrinology and metabolism,
M Miyazaki, and H Shirataki, and H Kohno, and K Kaibuchi, and A Tsugita, and Y Takai
Cullin-associated NEDD8-dissociated protein 1, a novel interactor of rabphilin-3A, deubiquitylates rabphilin-3A and regulates arginine vasopressin secretion in PC12 cells.
March 2018, Endocrine journal,
M Miyazaki, and H Shirataki, and H Kohno, and K Kaibuchi, and A Tsugita, and Y Takai
Purification and properties of Rabphilin-3A.
January 1995, Methods in enzymology,
M Miyazaki, and H Shirataki, and H Kohno, and K Kaibuchi, and A Tsugita, and Y Takai
Phosphorylation of Rabphilin-3A by calmodulin-dependent protein kinase II.
December 1994, Biochemical and biophysical research communications,
M Miyazaki, and H Shirataki, and H Kohno, and K Kaibuchi, and A Tsugita, and Y Takai
Rab3A GTPase-activating protein-inhibiting activity of Rabphilin-3A, a putative Rab3A target protein.
October 1993, The Journal of biological chemistry,
M Miyazaki, and H Shirataki, and H Kohno, and K Kaibuchi, and A Tsugita, and Y Takai
Structural determinants for Ca2+ and phosphatidylinositol 4,5-bisphosphate binding by the C2A domain of rabphilin-3A.
December 2008, The Journal of biological chemistry,
Copied contents to your clipboard!