Biomaterial Database

cDNA clones for salt-inducible genes from mustard (Brassica juncea). 1994

A S Gurjar, and P Roy

Hindustan Lever Research Centre, Bombay.

Treatment of a salt tolerant variety of mustard (Brassica juncea cv RH30), with 0.2M NaCl for 96 hr was found to induce synthesis of three new polypeptides and accumulation of proline. A cDNA library from mRNAs derived from salt stressed plants was constructed in the expression vector lambda gt11. Screening by differential hybridization and by salt stress specific antibodies gave two clones msc1 and msc2 (mustard salt clone) respectively. msc1 hybridized to transcripts from salt stressed mustard seedlings only after 48 hr of 0.2M NaCl stress as also to transcripts from drought stress induced by 10% PEG for 24 hr. msc 1 cross hybridized with msc2 clone. The results show the common mechanism of stress response as elicited in other plants.

UI	MeSH Term	Description	Entries
D001937	Brassica	A plant genus of the family Cruciferae. It contains many species and cultivars used as food including cabbage, cauliflower, broccoli, Brussel sprouts, kale, collard greens, MUSTARD PLANT; (B. alba, B. junica, and B. nigra), turnips (BRASSICA NAPUS) and rapeseed (BRASSICA RAPA).	Broccoli,Brussel Sprout,Cabbage,Cauliflower,Collard Green,Kale,Cabbages,Collard Greens
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D012965	Sodium Chloride	A ubiquitous sodium salt that is commonly used to season food.	Sodium Chloride, (22)Na,Sodium Chloride, (24)NaCl
D018076	DNA, Complementary	Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.	Complementary DNA,cDNA,cDNA Probes,Probes, cDNA
D018506	Gene Expression Regulation, Plant	Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.	Plant Gene Expression Regulation,Regulation of Gene Expression, Plant,Regulation, Gene Expression, Plant