BIOMAT Database Logo BIOMAT Database Logo

Isolation of yeast artificial chromosome clones from 54 polymorphic loci mapped with high odds on human chromosome 4. 1994

J B Fan, and J DeYoung, and R Lagacé, and R A Lina, and Z Xu, and J C Murray, and K H Buetow, and J Weissenbach, and R D Goold, and D R Cox
Department of Genetics, Stanford University, Palo Alto, CA 94304.

We constructed a yeast artificial chromosome (YAC) framework map of human chromosome 4 by screening a YAC library with 63 polymorphic DNA markers located on the chromosome. These genetic markers are from two framework meiotic maps that had previously been constructed by two research groups, and are placed on the two maps with odds for their order of 1000:1 or greater. In addition to isolating and determining the sizes of 141 YAC clones for 54 of these markers, we combined the two framework meiotic maps to produce a single integrated map. These combined maps and the YAC clones provide a set of extended DNA loci ordered at high odds that can be used to isolate additional polymorphic loci and genes, and to serve as a framework for obtaining a higher resolution physical map of the chromosome.

UI MeSH Term Description Entries
D008040 Genetic Linkage The co-inheritance of two or more non-allelic GENES due to their being located more or less closely on the same CHROMOSOME. Genetic Linkage Analysis,Linkage, Genetic,Analyses, Genetic Linkage,Analysis, Genetic Linkage,Genetic Linkage Analyses,Linkage Analyses, Genetic,Linkage Analysis, Genetic
D008540 Meiosis A type of CELL NUCLEUS division, occurring during maturation of the GERM CELLS. Two successive cell nucleus divisions following a single chromosome duplication (S PHASE) result in daughter cells with half the number of CHROMOSOMES as the parent cells. M Phase, Meiotic,Meiotic M Phase,M Phases, Meiotic,Meioses,Meiotic M Phases,Phase, Meiotic M,Phases, Meiotic M
D011110 Polymorphism, Genetic The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level. Gene Polymorphism,Genetic Polymorphism,Polymorphism (Genetics),Genetic Polymorphisms,Gene Polymorphisms,Polymorphism, Gene,Polymorphisms (Genetics),Polymorphisms, Gene,Polymorphisms, Genetic
D002874 Chromosome Mapping Any method used for determining the location of and relative distances between genes on a chromosome. Gene Mapping,Linkage Mapping,Genome Mapping,Chromosome Mappings,Gene Mappings,Genome Mappings,Linkage Mappings,Mapping, Chromosome,Mapping, Gene,Mapping, Genome,Mapping, Linkage,Mappings, Chromosome,Mappings, Gene,Mappings, Genome,Mappings, Linkage
D002894 Chromosomes, Human, Pair 4 A specific pair of GROUP B CHROMOSOMES of the human chromosome classification. Chromosome 4
D005819 Genetic Markers A phenotypically recognizable genetic trait which can be used to identify a genetic locus, a linkage group, or a recombination event. Chromosome Markers,DNA Markers,Markers, DNA,Markers, Genetic,Genetic Marker,Marker, Genetic,Chromosome Marker,DNA Marker,Marker, Chromosome,Marker, DNA,Markers, Chromosome
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D016324 Sequence Tagged Sites Short tracts of DNA sequence that are used as landmarks in GENOME mapping. In most instances, 200 to 500 base pairs of sequence define a Sequence Tagged Site (STS) that is operationally unique in the human genome (i.e., can be specifically detected by the polymerase chain reaction in the presence of all other genomic sequences). The overwhelming advantage of STSs over mapping landmarks defined in other ways is that the means of testing for the presence of a particular STS can be completely described as information in a database. Sequence-Tagged Sites,Sequence Tagged Site,Sequence-Tagged Site,Site, Sequence Tagged,Site, Sequence-Tagged,Sites, Sequence Tagged,Sites, Sequence-Tagged,Tagged Site, Sequence,Tagged Sites, Sequence
D018244 Chromosomes, Artificial, Yeast Chromosomes in which fragments of exogenous DNA ranging in length up to several hundred kilobase pairs have been cloned into yeast through ligation to vector sequences. These artificial chromosomes are used extensively in molecular biology for the construction of comprehensive genomic libraries of higher organisms. Artificial Chromosomes, Yeast,Yeast Artificial Chromosomes,Chromosomes, Yeast Artificial,YAC (Chromosome),YACs (Chromosomes),Artificial Chromosome, Yeast,Chromosome, Yeast Artificial,Yeast Artificial Chromosome

Related Publications

J B Fan, and J DeYoung, and R Lagacé, and R A Lina, and Z Xu, and J C Murray, and K H Buetow, and J Weissenbach, and R D Goold, and D R Cox
Characterization of four yeast artificial chromosome clones mapped to human chromosome 21q22.1 with eight markers.
August 1997, Molecules and cells,
J B Fan, and J DeYoung, and R Lagacé, and R A Lina, and Z Xu, and J C Murray, and K H Buetow, and J Weissenbach, and R D Goold, and D R Cox
A resource of mapped human bacterial artificial chromosome clones.
October 1999, Genome research,
J B Fan, and J DeYoung, and R Lagacé, and R A Lina, and Z Xu, and J C Murray, and K H Buetow, and J Weissenbach, and R D Goold, and D R Cox
Isolation of single-copy human genes from a library of yeast artificial chromosome clones.
June 1989, Science (New York, N.Y.),
J B Fan, and J DeYoung, and R Lagacé, and R A Lina, and Z Xu, and J C Murray, and K H Buetow, and J Weissenbach, and R D Goold, and D R Cox
Isolation of cDNA clones using yeast artificial chromosome probes.
July 1990, Nucleic acids research,
J B Fan, and J DeYoung, and R Lagacé, and R A Lina, and Z Xu, and J C Murray, and K H Buetow, and J Weissenbach, and R D Goold, and D R Cox
Integration of physical, genetic and cytogenetic maps of human chromosome 7: isolation and analysis of yeast artificial chromosome clones for 117 mapped genetic markers.
March 1994, Human molecular genetics,
J B Fan, and J DeYoung, and R Lagacé, and R A Lina, and Z Xu, and J C Murray, and K H Buetow, and J Weissenbach, and R D Goold, and D R Cox
Isolation and characterization of yeast artificial chromosome clones linking the HLA-B and HLA-C loci.
March 1991, Proceedings of the National Academy of Sciences of the United States of America,
J B Fan, and J DeYoung, and R Lagacé, and R A Lina, and Z Xu, and J C Murray, and K H Buetow, and J Weissenbach, and R D Goold, and D R Cox
Isolation of polymorphic DNA fragments from human chromosome 4.
February 1987, Nucleic acids research,
J B Fan, and J DeYoung, and R Lagacé, and R A Lina, and Z Xu, and J C Murray, and K H Buetow, and J Weissenbach, and R D Goold, and D R Cox
Isolation of coding sequences from yeast artificial chromosome (Yac). Clones by exon amplification.
January 1997, Methods in molecular biology (Clifton, N.J.),
J B Fan, and J DeYoung, and R Lagacé, and R A Lina, and Z Xu, and J C Murray, and K H Buetow, and J Weissenbach, and R D Goold, and D R Cox
Analysis of yeast artificial chromosome clones.
January 1992, Methods in molecular biology (Clifton, N.J.),
J B Fan, and J DeYoung, and R Lagacé, and R A Lina, and Z Xu, and J C Murray, and K H Buetow, and J Weissenbach, and R D Goold, and D R Cox
Regional localization of 725 human chromosome 7-specific yeast artificial chromosome clones.
July 1994, Genomics,
Copied contents to your clipboard!