Biomaterial Database

Effect of antifreeze proteins on the motility of ram spermatozoa. 1994

S R Payne, and J E Oliver, and G C Upreti

Meat Industry Research Institute of New Zealand, Hamilton.

The effect of the antifreeze peptide type I (AFP) from Winter flounder, Pseudopleuronectes americanus, and the antifreeze glycoprotein (AFGP) from Antarctic cod, Dissostichus mawsoni, was assessed on the motility of ram spermatozoa (Dorset and Dorset x Romney) after chilling (5 degrees C) and after freeze-thawing. During chilling, spermatozoal motility decreased significantly at an AFP or AFGP concentration of 0.1 microgram/ml and at concentrations above 10 micrograms/ml (P < 0.05). Thus, at 5 degrees C these antifreeze proteins can have a mildly cytotoxic effect at certain concentrations. Addition of AFP or AFGP to the freezing medium at concentrations of 0.1 to 10 micrograms/ml significantly reduced the loss in spermatozoal motility that occurs due to the freeze-thaw process (P < 0.001). The effect was not concentration dependent nor did it depend on which antifreeze protein was added. However, due to the cytotoxicity during the chilling stage, only AFP at a concentration of 10 micrograms/ml increased the percentage of motile spermatozoa significantly following freezing and thawing over that of the control (P < 0.05). Mechanisms to explain the effect of these proteins on spermatozoal motility after chilling and after freeze-thawing are proposed.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D003451	Cryoprotective Agents	Substances that provide protection against the harmful effects of freezing temperatures.	Cryoprotective Agent,Cryoprotective Effect,Cryoprotective Effects,Agent, Cryoprotective,Agents, Cryoprotective,Effect, Cryoprotective,Effects, Cryoprotective
D005069	Evaluation Studies as Topic	Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies.	Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D005399	Fishes	A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.
D005432	Flounder	Common name for two families of FLATFISHES belonging to the order Pleuronectiformes: left-eye flounders (Bothidae) and right-eye flounders (Pleuronectidae). The latter is more commonly used in research.	Plaice,Platichthys,Pleuronectes,Pseudopleuronectes,Halibut,Pleuronectes platessa,Flounders
D005615	Freezing	Liquids transforming into solids by the removal of heat.	Melting
D006023	Glycoproteins	Conjugated protein-carbohydrate compounds including MUCINS; mucoid, and AMYLOID glycoproteins.	C-Glycosylated Proteins,Glycosylated Protein,Glycosylated Proteins,N-Glycosylated Proteins,O-Glycosylated Proteins,Glycoprotein,Neoglycoproteins,Protein, Glycosylated,Proteins, C-Glycosylated,Proteins, Glycosylated,Proteins, N-Glycosylated,Proteins, O-Glycosylated
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012756	Sheep	Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.	Ovis,Sheep, Dall,Dall Sheep,Ovis dalli
D013081	Sperm Motility	Movement characteristics of SPERMATOZOA in a fresh specimen. It is measured as the percentage of sperms that are moving, and as the percentage of sperms with productive flagellar motion such as rapid, linear, and forward progression.	Motilities, Sperm,Motility, Sperm,Sperm Motilities