Biomaterial Database

Localization of the protective antigen in Salmonella typhimurium. 1976

A Mates, and H Yosipovici

Active protection to challenge with 100 LD50 of Salmonella typhimurium could be induced in white mice by inoculation with 10(3) live bacteria (0.1 LD50) or 10(8) heat killed bacteria (56 degrees C for 30 min), or 20 gamma ribosomal fraction. Passive protection could be given by antisera taken from hyperimmune rabbits inoculated with either the heat killed bacteria or by the ribosomal fraction. No direct correlation between antibody titre to 'O' antigen and degree of protection was observed. Absorption of any antisera with 'O' antigen decreases its protective power. The protective antibodies could be completely absorbed from both antisera by heat killed bacteria and released afterwards. Abosrption of 'O' antibodies from this preparation decreases the ability of the mice to survive the challenge. Our data suggests that the antigens which induce protective antibodies are located on the surface of the bacteria. The protection achieved by this antisera is probably the result of a synergistic effect between the antibodies to the 'O' antigen and other antigen(s).

UI	MeSH Term	Description	Entries
D007106	Immune Sera	Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.	Antisera,Immune Serums,Sera, Immune,Serums, Immune
D002473	Cell Wall	The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.	Cell Walls,Wall, Cell,Walls, Cell
D005260	Female		Females
D006358	Hot Temperature	Presence of warmth or heat or a temperature notably higher than an accustomed norm.	Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D000042	Absorption	The physical or physiological processes by which substances, tissue, cells, etc. take up or take in other substances or energy.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000907	Antibodies, Bacterial	Immunoglobulins produced in a response to BACTERIAL ANTIGENS.	Bacterial Antibodies
D000942	Antigens, Bacterial	Substances elaborated by bacteria that have antigenic activity.	Bacterial Antigen,Bacterial Antigens,Antigen, Bacterial
D001428	Bacterial Vaccines	Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.	Bacterial Vaccine,Bacterin,Vaccine, Bacterial,Vaccines, Bacterial
D012270	Ribosomes	Multicomponent ribonucleoprotein structures found in the CYTOPLASM of all cells, and in MITOCHONDRIA, and PLASTIDS. They function in PROTEIN BIOSYNTHESIS via GENETIC TRANSLATION.	Ribosome