BIOMAT Database Logo BIOMAT Database Logo

Somatic cell hybrid panel and NotI linking clones for physical mapping of human chromosome 3. 1994

J Y Wang, and E R Zabarovsky, and C Talmadge, and P Berglund, and K W Chan, and E S Pokrovskaya, and V I Kashuba, and D K Zhen, and F Boldog, and V I Zabarovskaya
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-5660.

To identify by positional cloning the putative tumor-suppressor genes on the short arm of human chromosome 3 that are involved in the initiation or progression of several human malignancies, we have developed human mouse somatic cell hybrids and NotI linking libraries. The somatic cell hybrids contain either the intact human chromosome 3 or its derivatives as the only human genetic material in rodent background. The somatic cell hybrid panel defines five chromosomal regions on the short arm and two chromosomal regions on the long arm of chromosome 3. Two hundred sixty-one NotI linking probes from three independently constructed linking libraries were assigned to the seven chromosomal regions. The somatic cell hybrid panel and the regionally localized NotI linking probes should facilitate the construction of genetic linkage and physical maps to identify various tumor-suppressor and disease-related genes not only on the chromosome 3p, but on the entire chromosome.

UI MeSH Term Description Entries
D008040 Genetic Linkage The co-inheritance of two or more non-allelic GENES due to their being located more or less closely on the same CHROMOSOME. Genetic Linkage Analysis,Linkage, Genetic,Analyses, Genetic Linkage,Analysis, Genetic Linkage,Genetic Linkage Analyses,Linkage Analyses, Genetic,Linkage Analysis, Genetic
D002872 Chromosome Deletion Actual loss of portion of a chromosome. Monosomy, Partial,Partial Monosomy,Deletion, Chromosome,Deletions, Chromosome,Monosomies, Partial,Partial Monosomies
D002874 Chromosome Mapping Any method used for determining the location of and relative distances between genes on a chromosome. Gene Mapping,Linkage Mapping,Genome Mapping,Chromosome Mappings,Gene Mappings,Genome Mappings,Linkage Mappings,Mapping, Chromosome,Mapping, Gene,Mapping, Genome,Mapping, Linkage,Mappings, Chromosome,Mappings, Gene,Mappings, Genome,Mappings, Linkage
D002893 Chromosomes, Human, Pair 3 A specific pair of human chromosomes in group A (CHROMOSOMES, HUMAN, 1-3) of the human chromosome classification. Chromosome 3
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006822 Hybrid Cells Any cell, other than a ZYGOTE, that contains elements (such as NUCLEI and CYTOPLASM) from two or more different cells, usually produced by artificial CELL FUSION. Somatic Cell Hybrids,Cell Hybrid, Somatic,Cell Hybrids, Somatic,Cell, Hybrid,Cells, Hybrid,Hybrid Cell,Hybrid, Somatic Cell,Hybrids, Somatic Cell,Somatic Cell Hybrid
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015183 Restriction Mapping Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA. Endonuclease Mapping, Restriction,Enzyme Mapping, Restriction,Site Mapping, Restriction,Analysis, Restriction Enzyme,Enzyme Analysis, Restriction,Restriction Enzyme Analysis,Analyses, Restriction Enzyme,Endonuclease Mappings, Restriction,Enzyme Analyses, Restriction,Enzyme Mappings, Restriction,Mapping, Restriction,Mapping, Restriction Endonuclease,Mapping, Restriction Enzyme,Mapping, Restriction Site,Mappings, Restriction,Mappings, Restriction Endonuclease,Mappings, Restriction Enzyme,Mappings, Restriction Site,Restriction Endonuclease Mapping,Restriction Endonuclease Mappings,Restriction Enzyme Analyses,Restriction Enzyme Mapping,Restriction Enzyme Mappings,Restriction Mappings,Restriction Site Mapping,Restriction Site Mappings,Site Mappings, Restriction

Related Publications

J Y Wang, and E R Zabarovsky, and C Talmadge, and P Berglund, and K W Chan, and E S Pokrovskaya, and V I Kashuba, and D K Zhen, and F Boldog, and V I Zabarovskaya
Development and utilization of a somatic cell hybrid mapping panel to assign NotI linking probes to the long arm of human chromosome 6.
March 1992, Genomics,
J Y Wang, and E R Zabarovsky, and C Talmadge, and P Berglund, and K W Chan, and E S Pokrovskaya, and V I Kashuba, and D K Zhen, and F Boldog, and V I Zabarovskaya
A somatic cell hybrid panel and DNA probes for physical mapping of human chromosome 7p.
November 1991, Genomics,
J Y Wang, and E R Zabarovsky, and C Talmadge, and P Berglund, and K W Chan, and E S Pokrovskaya, and V I Kashuba, and D K Zhen, and F Boldog, and V I Zabarovskaya
Mapping of 22 Notl linking clones on human chromosome 3 by polymerase chain reaction and somatic cell hybrid panels.
January 1996, Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology,
J Y Wang, and E R Zabarovsky, and C Talmadge, and P Berglund, and K W Chan, and E S Pokrovskaya, and V I Kashuba, and D K Zhen, and F Boldog, and V I Zabarovskaya
Development of a somatic cell hybrid mapping panel and molecular probes for human chromosome 3.
November 1990, Genomics,
J Y Wang, and E R Zabarovsky, and C Talmadge, and P Berglund, and K W Chan, and E S Pokrovskaya, and V I Kashuba, and D K Zhen, and F Boldog, and V I Zabarovskaya
Isolation and mapping of 45 NotI linking clones to chromosome 22.
September 1993, Genomics,
J Y Wang, and E R Zabarovsky, and C Talmadge, and P Berglund, and K W Chan, and E S Pokrovskaya, and V I Kashuba, and D K Zhen, and F Boldog, and V I Zabarovskaya
Analysis of NotI linking clones isolated from human chromosome 3 specific libraries.
November 1999, Gene,
J Y Wang, and E R Zabarovsky, and C Talmadge, and P Berglund, and K W Chan, and E S Pokrovskaya, and V I Kashuba, and D K Zhen, and F Boldog, and V I Zabarovskaya
A somatic cell hybrid panel for regional mapping of human chromosome 18.
October 1992, Genomics,
J Y Wang, and E R Zabarovsky, and C Talmadge, and P Berglund, and K W Chan, and E S Pokrovskaya, and V I Kashuba, and D K Zhen, and F Boldog, and V I Zabarovskaya
DNA sequence analysis of human chromosome 21 notI linking clones.
November 1993, Genomics,
J Y Wang, and E R Zabarovsky, and C Talmadge, and P Berglund, and K W Chan, and E S Pokrovskaya, and V I Kashuba, and D K Zhen, and F Boldog, and V I Zabarovskaya
Localization of 616 human chromosome 3-specific cosmids using a somatic cell hybrid deletion mapping panel.
September 1991, Genomics,
J Y Wang, and E R Zabarovsky, and C Talmadge, and P Berglund, and K W Chan, and E S Pokrovskaya, and V I Kashuba, and D K Zhen, and F Boldog, and V I Zabarovskaya
Regional localization of 188 sequence tagged sites on a somatic cell hybrid mapping panel for human chromosome 3.
December 1994, Genomics,
Copied contents to your clipboard!