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Within the last decade a variety of techniques have been developed and used for the detection of cell-mediated immunity in man by means of leucocyte migration inhibition in vitro. A detailed description of the leucocyte migration capillary tube technique (LMCT) and the leucocyte migration agarose technique (LMAT) is given. The procedure for selecting and using the proper antigen concentration is described. A description is also given of the indirect LMAT and the technique for determination of concanavalin A-induced lymphocyte release of leucocyte migration inhibition factor. Applications of these techniques are mainly intended for the exploration of the immunobiology of lymphocytes and cellular interactions associated with the immune response and the investigation of clinical conditions in man, i.e. infectious diseases, autoimmune diseases, transplantation states, tumour diseases, contact hypersensitivity and immunological deficiency states. Selection and adaptation to suit the experimental aim is necessary to obtain optimal results with these techniques. Their usefulness may be increased through more extensive use of purified antigens and indirect assays.
G Bendixen, and
K Bendtzen, and
J E Calusen, and
M Kjaer, and
M Søborg
December 1983,
The Journal of tropical medicine and hygiene,
G Bendixen, and
K Bendtzen, and
J E Calusen, and
M Kjaer, and
M Søborg
January 1974,
Journal of immunological methods,
G Bendixen, and
K Bendtzen, and
J E Calusen, and
M Kjaer, and
M Søborg
June 1973,
Clinical and experimental immunology,
G Bendixen, and
K Bendtzen, and
J E Calusen, and
M Kjaer, and
M Søborg
June 1969,
Lancet (London, England),
G Bendixen, and
K Bendtzen, and
J E Calusen, and
M Kjaer, and
M Søborg
January 1990,
Immunopharmacology and immunotoxicology,
G Bendixen, and
K Bendtzen, and
J E Calusen, and
M Kjaer, and
M Søborg
October 1974,
Journal of clinical pathology,
G Bendixen, and
K Bendtzen, and
J E Calusen, and
M Kjaer, and
M Søborg
January 1979,
Dermatologica,
G Bendixen, and
K Bendtzen, and
J E Calusen, and
M Kjaer, and
M Søborg
August 1971,
Lancet (London, England),
G Bendixen, and
K Bendtzen, and
J E Calusen, and
M Kjaer, and
M Søborg
November 1982,
Veterinary microbiology,
G Bendixen, and
K Bendtzen, and
J E Calusen, and
M Kjaer, and
M Søborg
October 1980,
Irish journal of medical science,
