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Two fused genes were constructed which encode for two chimeric proteins in which either 10 or 191 N-terminal amino acids of mature mitochondrial aspartate aminotransferase had been attached to the entire polypeptide chain of cytosolic dihydrofolate reductase. The precursor and mature form of mitochondrial aspartate aminotransferase, dihydrofolate reductase and both chimeric proteins were synthesized in vitro and their import into isolated mitochondria was studied. Both chimeric proteins were taken up by isolated organelles, where they became protease resistant, thus indicating the ability of the N-terminal portion of the mature moiety of the precursor of mitochondrial aspartate aminotransferase to direct cytosolic dihydrofolate reductase into mitochondria.
S Giannattasio, and
A Azzariti, and
E Marra, and
E Quagliariello
December 1984,
The EMBO journal,
S Giannattasio, and
A Azzariti, and
E Marra, and
E Quagliariello
July 1990,
Biochemical and biophysical research communications,
S Giannattasio, and
A Azzariti, and
E Marra, and
E Quagliariello
January 1986,
The Journal of biological chemistry,
S Giannattasio, and
A Azzariti, and
E Marra, and
E Quagliariello
June 1985,
The Biochemical journal,
S Giannattasio, and
A Azzariti, and
E Marra, and
E Quagliariello
May 2004,
FEBS letters,
S Giannattasio, and
A Azzariti, and
E Marra, and
E Quagliariello
December 1984,
FEBS letters,
S Giannattasio, and
A Azzariti, and
E Marra, and
E Quagliariello
November 1991,
Archives of biochemistry and biophysics,
S Giannattasio, and
A Azzariti, and
E Marra, and
E Quagliariello
January 1990,
Journal of biochemistry,
S Giannattasio, and
A Azzariti, and
E Marra, and
E Quagliariello
November 1992,
Archives of biochemistry and biophysics,
S Giannattasio, and
A Azzariti, and
E Marra, and
E Quagliariello
July 1979,
Archives of biochemistry and biophysics,
