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This study demonstrates active production of adrenomedullin (AM) in cultured vascular endothelial cells (ECs). To identify the origin of plasma AM and its functional relationship to vascular smooth muscle cells (VSMCs), we checked production of AM in a series of tissues and cell lines and found immunoreactive (ir-) AM in culture media of rat, porcine, human and bovine ECs. Ir-AM was accumulated linearly for up to 48 hours in the culture medium of rat ECs, and the secretion rate of AM was almost comparable to that of endothelin-1. By gel filtration and reverse phase high performance liquid chromatography, ir-AM in the culture medium was shown to have chromatographic behavior indistinguishable from that of synthetic rat AM. By RNA blot analysis of rat tissue, the most highly positive band was detected in cultured ECs, at an intensity 20 to 40 fold higher than that in adrenal gland. Based on these data as well as the presence of AM specific receptor on VSMCs, AM secreted from ECs is deduced to act directly on VSMCs, regulating vascular tone.
S Sugo, and
N Minamino, and
K Kangawa, and
K Miyamoto, and
K Kitamura, and
J Sakata, and
T Eto, and
H Matsuo
March 2000,
FEBS letters,
S Sugo, and
N Minamino, and
K Kangawa, and
K Miyamoto, and
K Kitamura, and
J Sakata, and
T Eto, and
H Matsuo
June 1990,
The American journal of pathology,
S Sugo, and
N Minamino, and
K Kangawa, and
K Miyamoto, and
K Kitamura, and
J Sakata, and
T Eto, and
H Matsuo
January 1987,
Hepatology (Baltimore, Md.),
S Sugo, and
N Minamino, and
K Kangawa, and
K Miyamoto, and
K Kitamura, and
J Sakata, and
T Eto, and
H Matsuo
March 2012,
Anatomy & cell biology,
S Sugo, and
N Minamino, and
K Kangawa, and
K Miyamoto, and
K Kitamura, and
J Sakata, and
T Eto, and
H Matsuo
October 1980,
The Journal of biological chemistry,
S Sugo, and
N Minamino, and
K Kangawa, and
K Miyamoto, and
K Kitamura, and
J Sakata, and
T Eto, and
H Matsuo
October 1992,
Japanese journal of cancer research : Gann,
S Sugo, and
N Minamino, and
K Kangawa, and
K Miyamoto, and
K Kitamura, and
J Sakata, and
T Eto, and
H Matsuo
June 1983,
Biology of reproduction,
S Sugo, and
N Minamino, and
K Kangawa, and
K Miyamoto, and
K Kitamura, and
J Sakata, and
T Eto, and
H Matsuo
June 1998,
Proceedings of the National Academy of Sciences of the United States of America,
S Sugo, and
N Minamino, and
K Kangawa, and
K Miyamoto, and
K Kitamura, and
J Sakata, and
T Eto, and
H Matsuo
May 1994,
Hepatology (Baltimore, Md.),
S Sugo, and
N Minamino, and
K Kangawa, and
K Miyamoto, and
K Kitamura, and
J Sakata, and
T Eto, and
H Matsuo
January 2022,
Cancer discovery,
