BIOMAT Database Logo BIOMAT Database Logo

Identification of a calcium dependent atrial secretory binding protein as calmodulin. 1994

A F Doubell, and C Lazure, and J Tremblay, and G Thibault
Department of Internal Medicine, Faculty of Medicine, University of Stellenbosch, Tygerberg, South Africa.

OBJECTIVE The role of calcium in the regulation of atrial natriuretic factor (ANF) secretion has focused attention on calcium binding proteins as regulatory components of the secretory machinery for ANF. Two members of the annexin family of calcium binding proteins have emerged as candidates for this role. The aim of this paper is to report the isolation, purification, and identification of another calcium dependent atrial secretory granule binding protein. METHODS A 20 kDa calcium binding protein was isolated from rat cardiac tissue by affinity chromatography and purified by ion exchange high pressure liquid chromatography followed by reverse phase high pressure liquid chromatography. Identification of the protein was based on its electrophoretic mobility, chromatographic properties, amino acid composition, and ability to stimulate the activity of calmodulin dependent phosphodiesterase. RESULTS Analysis of this protein and its interaction with atrial secretory granules revealed that it was identical to calmodulin and that the calcium dependent interaction with atrial secretory granules takes place at physiological calcium concentrations. CONCLUSIONS The study identifies calmodulin as a calcium dependent atrial secretory binding protein. The interaction of calmodulin with atrial secretory granules at physiological calcium concentrations suggest a possible role for calmodulin in the regulation of ANF release from the secretory granules.

UI MeSH Term Description Entries
D009206 Myocardium The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow. Muscle, Cardiac,Muscle, Heart,Cardiac Muscle,Myocardia,Cardiac Muscles,Heart Muscle,Heart Muscles,Muscles, Cardiac,Muscles, Heart
D009320 Atrial Natriuretic Factor A potent natriuretic and vasodilatory peptide or mixture of different-sized low molecular weight PEPTIDES derived from a common precursor and secreted mainly by the HEART ATRIUM. All these peptides share a sequence of about 20 AMINO ACIDS. ANF,ANP,Atrial Natriuretic Peptide,Atrial Natriuretic Peptides,Atriopeptins,Auriculin,Natriuretic Peptides, Atrial,ANF (1-126),ANF (1-28),ANF (99-126),ANF Precursors,ANP (1-126),ANP (1-28),ANP Prohormone (99-126),ANP-(99-126),Atrial Natriuretic Factor (1-126),Atrial Natriuretic Factor (1-28),Atrial Natriuretic Factor (99-126),Atrial Natriuretic Factor Precursors,Atrial Natriuretic Factor Prohormone,Atrial Natriuretic Peptide (1-126),Atrial Pronatriodilatin,Atriopeptigen,Atriopeptin (1-28),Atriopeptin (99-126),Atriopeptin 126,Atriopeptin Prohormone (1-126),Cardiodilatin (99-126),Cardiodilatin Precursor,Cardionatrin I,Cardionatrin IV,Prepro-ANP,Prepro-CDD-ANF,Prepro-Cardiodilatin-Atrial Natriuretic Factor,Pro-ANF,ProANF,Proatrial Natriuretic Factor,Pronatriodilatin,alpha ANP,alpha-ANP Dimer,alpha-Atrial Natriuretic Peptide,beta-ANP,beta-Atrial Natriuretic Peptide,gamma ANP (99-126),gamma-Atrial Natriuretic Peptide,Natriuretic Peptide, Atrial,Peptide, Atrial Natriuretic,Peptides, Atrial Natriuretic,Prepro ANP,Prepro CDD ANF,Prepro Cardiodilatin Atrial Natriuretic Factor,Pro ANF,alpha ANP Dimer,alpha Atrial Natriuretic Peptide,beta ANP,beta Atrial Natriuretic Peptide,gamma Atrial Natriuretic Peptide
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002147 Calmodulin A heat-stable, low-molecular-weight activator protein found mainly in the brain and heart. The binding of calcium ions to this protein allows this protein to bind to cyclic nucleotide phosphodiesterases and to adenyl cyclase with subsequent activation. Thereby this protein modulates cyclic AMP and cyclic GMP levels. Calcium-Dependent Activator Protein,Calcium-Dependent Regulator,Bovine Activator Protein,Cyclic AMP-Phosphodiesterase Activator,Phosphodiesterase Activating Factor,Phosphodiesterase Activator Protein,Phosphodiesterase Protein Activator,Regulator, Calcium-Dependent,AMP-Phosphodiesterase Activator, Cyclic,Activating Factor, Phosphodiesterase,Activator Protein, Bovine,Activator Protein, Calcium-Dependent,Activator Protein, Phosphodiesterase,Activator, Cyclic AMP-Phosphodiesterase,Activator, Phosphodiesterase Protein,Calcium Dependent Activator Protein,Calcium Dependent Regulator,Cyclic AMP Phosphodiesterase Activator,Factor, Phosphodiesterase Activating,Protein Activator, Phosphodiesterase,Protein, Bovine Activator,Protein, Calcium-Dependent Activator,Protein, Phosphodiesterase Activator,Regulator, Calcium Dependent
D002846 Chromatography, Affinity A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Chromatography, Bioaffinity,Immunochromatography,Affinity Chromatography,Bioaffinity Chromatography
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D003594 Cytoplasmic Granules Condensed areas of cellular material that may be bounded by a membrane. Cytoplasmic Granule,Granule, Cytoplasmic,Granules, Cytoplasmic
D006325 Heart Atria The chambers of the heart, to which the BLOOD returns from the circulation. Heart Atrium,Left Atrium,Right Atrium,Atria, Heart,Atrium, Heart,Atrium, Left,Atrium, Right
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

Related Publications

A F Doubell, and C Lazure, and J Tremblay, and G Thibault
Identification of a calcium-dependent calmodulin-binding domain in Xenopus membrane skeleton protein 4.1.
July 1991, The Journal of biological chemistry,
A F Doubell, and C Lazure, and J Tremblay, and G Thibault
Calcium-dependent calmodulin binding to chromaffin granule membranes: presence of a 65-kilodalton calmodulin-binding protein.
February 1985, Journal of neurochemistry,
A F Doubell, and C Lazure, and J Tremblay, and G Thibault
Calcium ion binding properties of Medicago truncatula calcium/calmodulin-dependent protein kinase.
September 2012, Biochemistry,
A F Doubell, and C Lazure, and J Tremblay, and G Thibault
Identification of a calcium-binding protein as a calcium-dependent regulator of brain adenylate cyclase.
January 1975, Proceedings of the National Academy of Sciences of the United States of America,
A F Doubell, and C Lazure, and J Tremblay, and G Thibault
Calcium/calmodulin-dependent protein kinase kinase: identification of regulatory domains.
October 1997, Biochemistry,
A F Doubell, and C Lazure, and J Tremblay, and G Thibault
Identification of rab12 as a secretory granule-associated small GTP-binding protein in atrial myocytes.
February 1996, Circulation research,
A F Doubell, and C Lazure, and J Tremblay, and G Thibault
Identification of a secretory granule-binding protein as caldesmon.
January 1986, Nature,
A F Doubell, and C Lazure, and J Tremblay, and G Thibault
Identification of the major postsynaptic density protein as homologous with the major calmodulin-binding subunit of a calmodulin-dependent protein kinase.
April 1984, Journal of neurochemistry,
A F Doubell, and C Lazure, and J Tremblay, and G Thibault
Protein p67. A calcium-binding protein localized at the sarcolemma of secretory atrial myocytes.
February 1992, Circulation research,
A F Doubell, and C Lazure, and J Tremblay, and G Thibault
Calcium/calmodulin-dependent protein kinases.
August 1994, Seminars in cancer biology,
Copied contents to your clipboard!