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The activity was studied of enkephalinase (endopeptidase 24.11, neutral endopeptidase)--a membrane enzyme of epithelial cells within human digestive tract (in the stomach, duodenum, small intestine, ascending, descending and sigmoid colon, and rectum). The enzyme activity was determined by column method using the labelled substrate (H-D-AlaLeu)--enkephalin and the selective enkephalin inhibitor--thiorfan in the presence of bestatin and captopril--inhibitors of aminopeptidases and angiotensin converting enzyme respectively. The highest enkephalinase activity was found in the duodenal epithelium (77.3 8.0 fmol of the substrate/min/mg of protein) and in the small intestine (23.4 0.9 fmol/min/mg of protein) with its gradual decrease when progressing down the gastrointestinal tract. This result confirms the hypothesis of enkephalinase participation in protein hydrolysis processes.
B Skryzdło-Radomańska, and
H Pollard, and
J Schabowski, and
P Radwan, and
K Celiński, and
J Pokora, and
C Gespach
January 1993,
Annales Universitatis Mariae Curie-Sklodowska. Sectio D: Medicina,
B Skryzdło-Radomańska, and
H Pollard, and
J Schabowski, and
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April 1997,
Journal of enzyme inhibition,
B Skryzdło-Radomańska, and
H Pollard, and
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January 1988,
Advances in experimental medicine and biology,
B Skryzdło-Radomańska, and
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K Celiński, and
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C Gespach
May 1993,
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies,
B Skryzdło-Radomańska, and
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J Schabowski, and
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Journal of medicinal chemistry,
B Skryzdło-Radomańska, and
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International journal of molecular medicine,
B Skryzdło-Radomańska, and
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K Celiński, and
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July 1991,
Neuropeptides,
B Skryzdło-Radomańska, and
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October 1995,
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas,
B Skryzdło-Radomańska, and
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C Gespach
February 1989,
Biochemical and biophysical research communications,
B Skryzdło-Radomańska, and
H Pollard, and
J Schabowski, and
P Radwan, and
K Celiński, and
J Pokora, and
C Gespach
March 1992,
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research,
