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A specific chemiluminescent method for the assessment of superoxide dismutase (SOD) activity in human blood has been developed. Intensive lucinogen-dependent chemiluminescence was observed during adrenalin oxidation in alkaline medium, and the level of this chemiluminescence essentially decreased in the presence of SOD. 50% inhibition of chemiluminescence was observed in the presence of 25 ng/ml of the enzyme, this indicating a high sensitivity of the method as against a similar spectrophotometric technique. SOD activities in donor blood samples were measured. The described method is offered for clinical application.
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