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Interleukin-1 beta (IL-1 beta) mediates a wide range of immune and inflammatory responses. The active cytokine is generated by proteolytic cleavage of an inactive precursor by a protease called the IL-1 beta converting enzyme (ICE). A cDNA encoding this protease was recently isolated. A human genomic clone containing the ICE gene (IL1BC) was isolated using the cDNA as a probe. The gene consists of 10 exons spanning at least 10.6 kb. 5'-anchored polymerase chain reaction indicated a single transcription start site approximately 33 bp upstream of the initiator Met codon. The 5'-flanking region does not have an apparent TATA box but may contain an initiator (Inr) promoter element. However, transcriptional activity could not be detected with a fusion gene containing the 5'-flanking region linked to the bacterial chloramphenicol acetyltransferase gene (CAT) when transfected into the human acute monocytic leukemia cell line THP-1. Using the genomic IL1BC clone, we have confirmed the localization of the gene to chromosome 11 band q22.2-q22.3 by fluorescence in situ hybridization.
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L T Hollingsworth, and
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M B Valentine, and
D N Shapiro, and
S W Morris, and
N Nelson
April 1992,
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N Nelson
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Zhonghua yi xue za zhi,
D P Cerretti, and
L T Hollingsworth, and
C J Kozlosky, and
M B Valentine, and
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N Nelson
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N Nelson
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D P Cerretti, and
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N Nelson
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D P Cerretti, and
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N Nelson
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M B Valentine, and
D N Shapiro, and
S W Morris, and
N Nelson
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D P Cerretti, and
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M B Valentine, and
D N Shapiro, and
S W Morris, and
N Nelson
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Journal of cellular biochemistry,
