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Organ transplantation in presensitized recipients continues to be contraindicated for heart and kidney recipients due to the risk of hyperacute rejection, which has no known treatment at this time. We tested whether donor serum, which contains soluble MHC class I antigen, is able to neutralize the effect of anti-donor antibody in the recipient and prevent hyperacute or accelerated rejection. A rat model of passive immunization was used to test the role of anti-donor antibody in hyperacute rejection. Seven of 10 recipients of hyperimmune serum (HyS), derived from Lewis rats (RT1l) following 3 ACI (RT1a) skin grafts, developed hyperacute or accelerated rejection. Intravenous injection of ACI serum prior to the HyS administration prevented hyperacute rejection in all recipients tested. When third-party (Wistar-Furth, RT1u) serum was given to Lewis rats injected with HyS, hyperacute rejection was not abrogated. When examining the mechanism of this effect, a simple antibody blocking phenomenon was found to be unlikely since flow cytometry analysis showed that ACI serum needed to be present at > or = 256-fold excess compared to HyS to block anti-ACI antibody binding to RT1.Aa+cells by 50%. We tested whether the RT1.Aa class I antigen in ACI serum had other biologic properties that resulted in the prolonged graft survival. However, removal of RT1.Aa antigen from ACI serum prior to use in the passive transfer model did not abrogate the graft prolongation observed previously. These data suggest that components of donor serum other than MHC class I antigen may be useful for preventing the antibody-mediated component of hyperacute rejection.
J Wang, and
E K Geissler, and
J H Fechner, and
W J Burlingham, and
S J Knechtle
October 1979,
Laboratory investigation; a journal of technical methods and pathology,
J Wang, and
E K Geissler, and
J H Fechner, and
W J Burlingham, and
S J Knechtle
November 1972,
Transplantation,
J Wang, and
E K Geissler, and
J H Fechner, and
W J Burlingham, and
S J Knechtle
August 1983,
Transplantation,
J Wang, and
E K Geissler, and
J H Fechner, and
W J Burlingham, and
S J Knechtle
June 1994,
Transplant immunology,
J Wang, and
E K Geissler, and
J H Fechner, and
W J Burlingham, and
S J Knechtle
April 1996,
Transplantation proceedings,
J Wang, and
E K Geissler, and
J H Fechner, and
W J Burlingham, and
S J Knechtle
January 1995,
Advances in nephrology from the Necker Hospital,
J Wang, and
E K Geissler, and
J H Fechner, and
W J Burlingham, and
S J Knechtle
October 1968,
Surgery,
J Wang, and
E K Geissler, and
J H Fechner, and
W J Burlingham, and
S J Knechtle
February 1987,
Transplantation proceedings,
J Wang, and
E K Geissler, and
J H Fechner, and
W J Burlingham, and
S J Knechtle
July 1984,
Transplantation,
J Wang, and
E K Geissler, and
J H Fechner, and
W J Burlingham, and
S J Knechtle
June 1980,
Transplantation,
