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A subpopulation of corticotropin-releasing hormone neurosecretory cells in the paraventricular nucleus of the hypothalamus also contain NADPH-diaphorase. 1994
P Siaud, and
M Mekaouche, and
G Ixart, and
M Balmefrezol, and
L Givalois, and
G Barbanel, and
I Assenmacher
Laboratory of Endocrinological Neurobiology, URA 1197 CNRS, University of Montepellier II, France.
The coexistence of ND with CRH 41 was explored in the parvicellular neurons of the PVN, using dual histochemical and radioimmunocytochemical labelling with the light microscope, in rats treated with colchicine. Even though the ND staining was scarce, a clear colocalization was evidenced in the parvicellular part of the PVN. Under these conditions, the ratio of neurons expressing both markers, ND and CRH, amounted about 15% of the CRH-containing neuron population. This result provides a useful tool to study morphological plastic changes in the PVN in response to environmental variations.
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