BIOMAT Database Logo BIOMAT Database Logo

Frog diazepam-binding inhibitor: peptide sequence, cDNA cloning, and expression in the brain. 1994

I Lihrmann, and J C Plaquevent, and H Tostivint, and R Raijmakers, and M C Tonon, and J M Conlon, and H Vaudry
European Institute for Peptide Research, Institut National de la Santé de la Recherche Médicale Unité 413, University of Rouen, Mont-Saint-Aignan, France.

Three peptides derived from diazepam-binding inhibitor (DBI) were isolated in pure form from the brain of the frog Rana ridibunda. The primary structures of these peptides showed that they correspond to mammalian DBI-(1-39), DBI-(58-87), and DBI-(70-87). A set of degenerate primers, whose design was based on the amino acid sequence data, was used to screen a frog brain cDNA library. The cloned cDNA encodes an 87-amino acid polypeptide, which exhibits 68% similarity with porcine and bovine DBI. Frog DBI contains two paired basic amino acids (Lys-Lys) at positions 14-15 and 62-63 and a single cysteine within the biologically active region of the molecule. Northern blot analysis showed that DBI mRNA is expressed at a high level in the brain but is virtually absent in peripheral tissues. The distribution of DBI mRNA and DBI-like immunoreactivity in the frog brain was studied by in situ hybridization and immunocytochemistry. Both approaches revealed that the DBI gene is expressed in ependymal cells and circumventricular organs lining the ventricular cavity. Since amphibia diverged from mammals at least 250 million years ago, the data show that evolutionary pressure has acted to conserve the structure of DBI in the vertebrate phylum. The distribution of both DBI mRNA and DBI-like immunoreactivity indicates that DBI is selectively expressed in glial cells.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D011895 Rana ridibunda A species of the family Ranidae which occurs primarily in Europe and is used widely in biomedical research.
D001921 Brain The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM. Encephalon
D002352 Carrier Proteins Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes. Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D014018 Tissue Distribution Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios. Distribution, Tissue,Distributions, Tissue,Tissue Distributions

Related Publications

I Lihrmann, and J C Plaquevent, and H Tostivint, and R Raijmakers, and M C Tonon, and J M Conlon, and H Vaudry
Diazepam binding inhibitor peptide: cloning and gene expression.
December 1991, Neuropharmacology,
I Lihrmann, and J C Plaquevent, and H Tostivint, and R Raijmakers, and M C Tonon, and J M Conlon, and H Vaudry
Cloning and Sequencing of cDNA Encoding the Diazepam Binding Inhibitor (DBI).
January 1998, Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica,
I Lihrmann, and J C Plaquevent, and H Tostivint, and R Raijmakers, and M C Tonon, and J M Conlon, and H Vaudry
Molecular cloning and expression of a novel human cDNA related to the diazepam binding inhibitor.
May 1999, Biochimica et biophysica acta,
I Lihrmann, and J C Plaquevent, and H Tostivint, and R Raijmakers, and M C Tonon, and J M Conlon, and H Vaudry
Putative diazepam binding inhibitor peptide: cDNA clones from rat.
October 1986, Proceedings of the National Academy of Sciences of the United States of America,
I Lihrmann, and J C Plaquevent, and H Tostivint, and R Raijmakers, and M C Tonon, and J M Conlon, and H Vaudry
Sequence and expression of the murine diazepam binding inhibitor.
November 1989, Brain research. Molecular brain research,
I Lihrmann, and J C Plaquevent, and H Tostivint, and R Raijmakers, and M C Tonon, and J M Conlon, and H Vaudry
Carp cDNA sequence encoding a putative diazepam-binding inhibitor/endozepine/acyl-CoA-binding protein.
November 1996, Biochimica et biophysica acta,
I Lihrmann, and J C Plaquevent, and H Tostivint, and R Raijmakers, and M C Tonon, and J M Conlon, and H Vaudry
Cloning and expression of frog rhodopsin cDNA.
March 1995, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,
I Lihrmann, and J C Plaquevent, and H Tostivint, and R Raijmakers, and M C Tonon, and J M Conlon, and H Vaudry
Increased expression of diazepam binding inhibitor in human brain tumors.
March 1995, Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research,
I Lihrmann, and J C Plaquevent, and H Tostivint, and R Raijmakers, and M C Tonon, and J M Conlon, and H Vaudry
Molecular biology of diazepam binding inhibitor peptide.
February 1990, Neurochemical research,
I Lihrmann, and J C Plaquevent, and H Tostivint, and R Raijmakers, and M C Tonon, and J M Conlon, and H Vaudry
Cloning and sequence analysis of cDNA encoding a precursor for rat brain natriuretic peptide.
March 1989, Biochemical and biophysical research communications,
Copied contents to your clipboard!