Biomaterial Database

[Age-related alterations on GTP binding proteins in postmortem human brain]. 1994

E Hashimoto, and H Ozawa, and T Saito

Department of Neuropsychiatry, School of Medicine, Sapporo Medical University, Japan.

It is important that natural aging-related alterations are characterized to understand disabilities associated with aging. In this study, age-related alterations of GTP binding (G) proteins were examined in membrane preparations from several regions (frontal cortex, temporal cortex, parietal cortex, occipital cortex, caudate nucleus, amygdaloid body) in postmortem human brain. Subjects were free from neurologic or psychiatric disease. The quantity of G proteins (Gs alpha, Gi alpha, Go alpha, Gq alpha, G beta subunit) was determined by immunoblotting with polyclonal antibody (RM/1, AS/7, GC/2, QL, SW/1, respectively). The function of G proteins was examined by photoaffinity GTP analog [azidoanilido GTP (AAGTP)] binding. The immunoreactivities of Gi alpha and Gq alpha subunits were correlated inversely with age in many areas. In caudate nucleus, the GsL alpha (45 kDa) and Go alpha subunit immunoreactivities were decreased with age. And the G beta subunit immunoreactivities showed a negative correlation with age in temporal and occipital cortex areas. However, AAGTP labeling to Gs alpha and Gi/o alpha and the ratio of Gs alpha to Gi/o alpha AAGTP binding showed no age-dependent changes. Our findings suggest that the functional modulation may compensate for quantitative alterations of G proteins with human senescence.

UI	MeSH Term	Description	Entries
D011180	Postmortem Changes	Physiological changes that occur in bodies after death.	Adipocere,Algor Mortis,Cruor,Livor Mortis,Change, Postmortem,Changes, Postmortem,Postmortem Change
D011485	Protein Binding	The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.	Plasma Protein Binding Capacity,Binding, Protein
D001921	Brain	The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.	Encephalon
D006160	Guanosine Triphosphate	Guanosine 5'-(tetrahydrogen triphosphate). A guanine nucleotide containing three phosphate groups esterified to the sugar moiety.	GTP,Triphosphate, Guanosine
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000375	Aging	The gradual irreversible changes in structure and function of an organism that occur as a result of the passage of time.	Senescence,Aging, Biological,Biological Aging
D001386	Azides	Organic or inorganic compounds that contain the -N3 group.	Azide
D019204	GTP-Binding Proteins	Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.	G-Proteins,GTP-Regulatory Proteins,Guanine Nucleotide Regulatory Proteins,G-Protein,GTP-Binding Protein,GTP-Regulatory Protein,Guanine Nucleotide Coupling Protein,G Protein,G Proteins,GTP Binding Protein,GTP Binding Proteins,GTP Regulatory Protein,GTP Regulatory Proteins,Protein, GTP-Binding,Protein, GTP-Regulatory,Proteins, GTP-Binding,Proteins, GTP-Regulatory