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The rhizocticines and plumbemicines are two groups of di- and tripeptid antibiotics thought to interfere with threonine or threonine-related metabolism. Z-2-amino-5-phosphono-3-pentenoic acid, the common unusual amino acid constituent of the rhizocticines and plumbemicines, was found to irreversibly inhibit Escherichia coli threonine synthase in a time-dependent reaction that followed pseudo-first order and saturation kinetics. These data provide evidence that the toxicity of the rhizocticines and plumbemicines is due to the inhibition of threonine synthase by Z-2-amino-5-phosphone-3-pentenoic acid, which is liberated by peptidases after uptake into the target cell. Additionally, methods for the purification of threonine synthase from an overproducing E. coli strain and for the enzymatic synthesis of L-homoserine phosphate are described.
B Laber, and
S D Lindell, and
H D Pohlenz
September 1995,
The Journal of antibiotics,
B Laber, and
S D Lindell, and
H D Pohlenz
June 1997,
Arzneimittel-Forschung,
B Laber, and
S D Lindell, and
H D Pohlenz
February 1997,
Arzneimittel-Forschung,
B Laber, and
S D Lindell, and
H D Pohlenz
June 1997,
Arzneimittel-Forschung,
B Laber, and
S D Lindell, and
H D Pohlenz
April 1990,
The Journal of biological chemistry,
B Laber, and
S D Lindell, and
H D Pohlenz
November 1993,
Journal of medicinal chemistry,
B Laber, and
S D Lindell, and
H D Pohlenz
June 1984,
Biochemistry,
B Laber, and
S D Lindell, and
H D Pohlenz
May 1986,
FEBS letters,
B Laber, and
S D Lindell, and
H D Pohlenz
May 1988,
Biochemistry,
B Laber, and
S D Lindell, and
H D Pohlenz
November 1963,
Archives of biochemistry and biophysics,
