-transparent.png){kind=logo}
In order to induce the production of antibodies specific for small molecules, it is common to link them to a protein. However, when the small molecule is very hydrophobic it is extremely difficult to prepare such a conjugate. Here, we describe a simple way to obtain an antigenic conjugate under controlled conditions: in a first step a very hydrophobic hapten, cholanic acid, is linked to a dye, basilen blue, in organic solvent; in a second step the cholanic acid-basilen blue conjugate is dissolved in phosphate buffered saline and mixed with rabbit polyclonal anti-basilen blue antibodies previously raised in rabbits against basilen blue-key-hole limpet hemocyanin conjugate. Such a complex, which dissociates very slowly, appears to be a good immunogen in mice. Anti-cholanyl residue monoclonal antibodies were produced and characterized.
K Chevrie, and
G Motta, and
R Mayer, and
M Monsigny
March 1985,
European journal of immunology,
K Chevrie, and
G Motta, and
R Mayer, and
M Monsigny
August 1988,
Molecular immunology,
K Chevrie, and
G Motta, and
R Mayer, and
M Monsigny
January 1981,
Journal of immunological methods,
K Chevrie, and
G Motta, and
R Mayer, and
M Monsigny
June 1985,
Journal of immunology (Baltimore, Md. : 1950),
K Chevrie, and
G Motta, and
R Mayer, and
M Monsigny
January 2014,
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment,
K Chevrie, and
G Motta, and
R Mayer, and
M Monsigny
January 1986,
Journal of immunology (Baltimore, Md. : 1950),
K Chevrie, and
G Motta, and
R Mayer, and
M Monsigny
January 1995,
Biotechnology progress,
K Chevrie, and
G Motta, and
R Mayer, and
M Monsigny
January 1975,
Journal of immunology (Baltimore, Md. : 1950),
K Chevrie, and
G Motta, and
R Mayer, and
M Monsigny
July 1987,
Molecular immunology,
K Chevrie, and
G Motta, and
R Mayer, and
M Monsigny
June 2009,
Hybridoma (2005),
