Biomaterial Database

[Controlled-expression vector for Pseudomonas bacteria involving regulatory elements of trpIBA genes of Pseudomonas putida]. 1994

I N Olekhnovich, and Iu K Fomichev

A bireplicon controlled-expression vector pPS10 was developed based, on trpIBA genes of Pseudomonas putida. It is a low-copy-number vector in Pseudomonas bacteria, and a high-copy-number vector in Escherichia coli. The vector is 10.4 kilobase pairs (kb), determines resistance to kanamycin, carries a replicon of cryptic Pseudomonas pMK1 plasmid; a pBR322 replicon; the par locus of pMT2 plasmid; and the trpI gene of P. putida, which encodes the activator protein and the promoter Pba of trpBA genes. Expression of the promoter is induced by the TrpI protein activator and the precursor of tryptophan, indole-3-glycerolphosphate (InGP). InGP is an unstable compound, and its accumulation in bacterial cells is ensured by using trpE mutants grown in the presence of anthranilate; no InGP is produced among trpE mutants on the media supplemented with tryptophan. As shown in the pheA gene of E. coli, the expression of genetic material cloned under control of the Pba promoter into the pPS10 vector, may be enhanced more than 70-fold in cells of Pseudomonas under conditions of InGP accumulation.

UI	MeSH Term	Description	Entries
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D011401	Promoter Regions, Genetic	DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.	rRNA Promoter,Early Promoters, Genetic,Late Promoters, Genetic,Middle Promoters, Genetic,Promoter Regions,Promoter, Genetic,Promotor Regions,Promotor, Genetic,Pseudopromoter, Genetic,Early Promoter, Genetic,Genetic Late Promoter,Genetic Middle Promoters,Genetic Promoter,Genetic Promoter Region,Genetic Promoter Regions,Genetic Promoters,Genetic Promotor,Genetic Promotors,Genetic Pseudopromoter,Genetic Pseudopromoters,Late Promoter, Genetic,Middle Promoter, Genetic,Promoter Region,Promoter Region, Genetic,Promoter, Genetic Early,Promoter, rRNA,Promoters, Genetic,Promoters, Genetic Middle,Promoters, rRNA,Promotor Region,Promotors, Genetic,Pseudopromoters, Genetic,Region, Genetic Promoter,Region, Promoter,Region, Promotor,Regions, Genetic Promoter,Regions, Promoter,Regions, Promotor,rRNA Promoters
D012093	Replicon	Any DNA sequence capable of independent replication or a molecule that possesses a REPLICATION ORIGIN and which is therefore potentially capable of being replicated in a suitable cell. (Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)	Replication Unit,Replication Units,Replicons,Unit, Replication,Units, Replication
D005809	Genes, Regulator	Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions.	Gene, Regulator,Regulator Gene,Regulator Genes,Regulatory Genes,Gene, Regulatory,Genes, Regulatory,Regulatory Gene
D005815	Genetic Code	The meaning ascribed to the BASE SEQUENCE with respect to how it is translated into AMINO ACID SEQUENCE. The start, stop, and order of amino acids of a protein is specified by consecutive triplets of nucleotides called codons (CODON).	Code, Genetic,Codes, Genetic,Genetic Codes
D005822	Genetic Vectors	DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.	Cloning Vectors,Shuttle Vectors,Vectors, Genetic,Cloning Vector,Genetic Vector,Shuttle Vector,Vector, Cloning,Vector, Genetic,Vector, Shuttle,Vectors, Cloning,Vectors, Shuttle
D015964	Gene Expression Regulation, Bacterial	Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.	Bacterial Gene Expression Regulation,Regulation of Gene Expression, Bacterial,Regulation, Gene Expression, Bacterial
D016958	Pseudomonas putida	A species of gram-negative, aerobic bacteria isolated from soil and water as well as clinical specimens. Occasionally it is an opportunistic pathogen.