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Rabbit antiserum against highly purified reaction center preparations was shown to react specifically with a single component of chromatophore membranes from Rhodopseudomonas spheroides strain R-26. The conjugate of purified gamma globulin and ferritin prepared with toluene diisocyanate was used to determine the localization of reaction centers in the chromatophore membranes. Virtually no antibody was bound by intact membranes. After removing the 9nm ATPase from these membranes by dilute EDTA treatment, a considerable amount of antibody was bound to the exposed outer membrane surface. The reaction center binding sites were estimated to be uniformly distributed with approx. 1 reaction center per 200 nm-2 of membrane surface. These results indicate that the reaction centers are located near the outer membrane surface but below the ATPase particles. Since the distribution of reaction centers and particles on rough faces seen by freeze-fracture particle may be a complex of a reaction center and other electron transfer components localized within the hydrophobic region of the membrane.
D W Reed, and
D Raveed, and
M Reporter
July 1979,
Biokhimiia (Moscow, Russia),
D W Reed, and
D Raveed, and
M Reporter
September 1973,
FEBS letters,
D W Reed, and
D Raveed, and
M Reporter
February 1972,
Biochimica et biophysica acta,
D W Reed, and
D Raveed, and
M Reporter
November 1971,
The Biochemical journal,
D W Reed, and
D Raveed, and
M Reporter
April 1973,
Biochimica et biophysica acta,
D W Reed, and
D Raveed, and
M Reporter
October 1964,
Nature,
D W Reed, and
D Raveed, and
M Reporter
January 1972,
Biophysical journal,
D W Reed, and
D Raveed, and
M Reporter
January 1978,
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae,
D W Reed, and
D Raveed, and
M Reporter
September 1969,
The Journal of biological chemistry,
D W Reed, and
D Raveed, and
M Reporter
April 1974,
Biochimica et biophysica acta,
