Biomaterial Database

Effect of several electrolyte preservation solutions on the water permeability of isolated cortical collecting ducts perfused in vitro. 1994

C A Ribeiro, and D Falkenstein, and J F Figueiredo

Department of Physiology and Biophysics, Faculty of Medical Sciences, State University of Campinas, São Paulo, Brazil.

The extent of nephron injury after long-term preservation is unknown. An experimental protocol was designed to study the effects of 1 and 24 h of cold storage in preservation solution on renal slices of rabbit cortical collecting duct (CCD) isolated and perfused in vitro and evaluated by water hydraulic conductivity. The preservation solutions were Ringer-bicarbonate, Collins, Euro-Collins, and University of Wisconsin (UW). Under these experimental preservation conditions, the following results were observed: after 1 h of preservation, the CCDs obtained from slices incubated in Ringer-bicarbonate, Collins, and Euro-Collins solutions at 4 degrees C presented a high basal hydraulic conductivity compared to the control group and responded well to arginine-vasopressin (AVP) water permeability stimulation. The CCDs derived from renal slices stored for 24 h in Ringer-bicarbonate solution at 4 degrees C showed no function, while those preserved in Collins and Euro-Collins solutions showed a lack of responsiveness to AVP water permeability stimulation. The CCDs preserved in UW solution showed better values than those preserved in the other solutions, although there was a decrease in hydraulic conductivity after 24 h of preservation. These data show that long-term cold preservation with flushed solutions impairs the cortical collecting duct response to AVP.

UI	MeSH Term	Description	Entries
D006982	Hypertonic Solutions	Solutions that have a greater osmotic pressure than a reference solution such as blood, plasma, or interstitial fluid.	Hypertonic Solution,Solution, Hypertonic,Solutions, Hypertonic
D007328	Insulin	A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).	Iletin,Insulin A Chain,Insulin B Chain,Insulin, Regular,Novolin,Sodium Insulin,Soluble Insulin,Chain, Insulin B,Insulin, Sodium,Insulin, Soluble,Regular Insulin
D007552	Isotonic Solutions	Solutions having the same osmotic pressure as blood serum, or another solution with which they are compared. (From Grant & Hackh's Chemical Dictionary, 5th ed & Dorland, 28th ed)	Solutions, Isotonic
D007672	Kidney Cortex	The outer zone of the KIDNEY, beneath the capsule, consisting of KIDNEY GLOMERULUS; KIDNEY TUBULES, DISTAL; and KIDNEY TUBULES, PROXIMAL.	Cortex, Kidney
D007685	Kidney Tubules, Collecting	Straight tubes commencing in the radiate part of the kidney cortex where they receive the curved ends of the distal convoluted tubules. In the medulla the collecting tubules of each pyramid converge to join a central tube (duct of Bellini) which opens on the summit of the papilla.	Kidney Collecting Ducts,Kidney Collecting Duct,Collecting Duct, Kidney,Collecting Ducts, Kidney
D009926	Organ Preservation	The process by which organs are kept viable outside of the organism from which they were removed (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).	Organ Preservations,Preservation, Organ,Preservations, Organ
D010477	Perfusion	Treatment process involving the injection of fluid into an organ or tissue.	Perfusions
D010539	Permeability	Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions.	Permeabilities
D011817	Rabbits	A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes.	Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D011887	Raffinose	A trisaccharide occurring in Australian manna (from Eucalyptus spp, Myrtaceae) and in cottonseed meal.	Gossypose,Melitose,Melitriose