Biomaterial Database

Sevoflurane-induced ionic current in acutely dissociated CA1 pyramidal neurons of the rat hippocampus. 1994

J Wu, and N Harata, and N Akaike

Department of Neurophysiology, Tohoku University School of Medicine, Sendai, Japan.

The electrophysiological properties of sevoflurane (Sev)-induced current (ISev) were investigated in CA1 pyramidal neurons freshly dissociated from the rat hippocampus by using the nystatin perforated patch recording configuration under voltage-clamp condition. Within the range of Sev concentrations from 3 x 10(-4) to 2 x 10(-3) M, ISev was an inward current which consisted of an initial transient peak component and a successive steady-state plateau component. The peak current component increased in a concentration-dependent manner with a conductance increase. The application of Sev over 2 x 10(-3) M, however, suppressed the peak and steady-state current components with a concomitant decrease in conductance and elicited a transient inward current ('hump' current) immediately after wash out. The current-voltage relationship for ISev showed some outward rectification suggesting a slight voltage-dependency of the ISev. The reversal potential of ISev (ESev) was close to the ECl and shifted by 52 mV for a 10-fold change in extracellular Cl- concentrations, indicating that ISev is passing through Cl- channels. The single channel conductance obtained from the analysis of the variance of ISev fluctuations was 15.3 +/- 1.3 pS.

UI	MeSH Term	Description	Entries
D007477	Ions	An atom or group of atoms that have a positive or negative electric charge due to a gain (negative charge) or loss (positive charge) of one or more electrons. Atoms with a positive charge are known as CATIONS; those with a negative charge are ANIONS.
D008738	Methyl Ethers	A group of compounds that contain the general formula R-OCH3.	Ethers, Methyl
D009994	Osmolar Concentration	The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.	Ionic Strength,Osmolality,Osmolarity,Concentration, Osmolar,Concentrations, Osmolar,Ionic Strengths,Osmolalities,Osmolar Concentrations,Osmolarities,Strength, Ionic,Strengths, Ionic
D002712	Chlorides	Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.	Chloride,Chloride Ion Level,Ion Level, Chloride,Level, Chloride Ion
D004305	Dose-Response Relationship, Drug	The relationship between the dose of an administered drug and the response of the organism to the drug.	Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D004553	Electric Conductivity	The ability of a substrate to allow the passage of ELECTRONS.	Electrical Conductivity,Conductivity, Electric,Conductivity, Electrical
D004560	Electricity	The physical effects involving the presence of electric charges at rest and in motion.
D004594	Electrophysiology	The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D004987	Ethers	Organic compounds having two alkyl or aryl groups bonded to an oxygen atom, as in the formula R1–O–R2.
D005110	Extracellular Space	Interstitial space between cells, occupied by INTERSTITIAL FLUID as well as amorphous and fibrous substances. For organisms with a CELL WALL, the extracellular space includes everything outside of the CELL MEMBRANE including the PERIPLASM and the cell wall.	Intercellular Space,Extracellular Spaces,Intercellular Spaces,Space, Extracellular,Space, Intercellular,Spaces, Extracellular,Spaces, Intercellular