Biomaterial Database

Removing the interference of DDT and its analogs in the analysis for residues of polychlorinated biphenyls. 1975

W J Trotter

A micro scale procedure is described for quantitatively separating polychlorinated biphenyls (PCBs) from DDT and its analogs. DDT and TDE are initially dehydrochlorinated with ethanolic KOH to their respective olefins, DDE and TDE-olefin (1-chloro-2,2-bis (p-chlorophenyl) ethylene). The olefins are than oxidized by CrO3 in acetic acid to the more polar dichlorobenzophenone. PCB's are eluted from a micro Florisil column with petroleum ether. Typical recoveries in the ppm range (based on a 5 g sample) were is greater than 80% for Aroclors 1254 and 1260. Recoveries generally were poorer with Aroclors of lower chlorine content and decreased with decreasing quantity of Aroclor present. The DDT group was not quantitatively determined; recoveries averaged is greater than 60% for the DDT group in the ppm range (based on a 5 g sample). The procedure was successfully applied to samples of Lake Michigan chubs containing residues of PCB's and the DDT group and to extracts of human serum fortified with Aroclors and the DDT group.

UI	MeSH Term	Description	Entries
D008832	Microchemistry	The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.
D010084	Oxidation-Reduction	A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).	Redox,Oxidation Reduction
D010573	Pesticide Residues	Pesticides or their breakdown products remaining in the environment following their normal use or accidental contamination.	Pesticide Residue,Residue, Pesticide,Residues, Pesticide
D011078	Polychlorinated Biphenyls	Industrial products consisting of a mixture of chlorinated biphenyl congeners and isomers. These compounds are highly lipophilic and tend to accumulate in fat stores of animals. Many of these compounds are considered toxic and potential environmental pollutants.	PCBs,Polychlorinated Biphenyl,Polychlorobiphenyl Compounds,Biphenyl, Polychlorinated,Biphenyls, Polychlorinated,Compounds, Polychlorobiphenyl
D002849	Chromatography, Gas	Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.	Chromatography, Gas-Liquid,Gas Chromatography,Chromatographies, Gas,Chromatographies, Gas-Liquid,Chromatography, Gas Liquid,Gas Chromatographies,Gas-Liquid Chromatographies,Gas-Liquid Chromatography
D003632	Dichlorodiphenyldichloroethane	An organochlorine insecticide that is slightly irritating to the skin. (From Merck Index, 11th ed, p482)	DDD,TDE
D003633	Dichlorodiphenyl Dichloroethylene	An organochlorine pesticide, it is the ethylene metabolite of DDT.	DDE,DDX,1,1-Dichloro-2,2-bis(p-chlorophenyl)ethylene,DDMU,p,p'-DDE,p,p-Dichlorodiphenyldichloroethylene,Dichloroethylene, Dichlorodiphenyl
D003634	DDT	A polychlorinated pesticide that is resistant to destruction by light and oxidation. Its unusual stability has resulted in difficulties in residue removal from water, soil, and foodstuffs. This substance may reasonably be anticipated to be a carcinogen: Fourth Annual Report on Carcinogens (NTP-85-002, 1985). (From Merck Index, 11th ed)	1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane,4,4'-DDT,4,4'-Dichlorodiphenyltrichloroethane,Benzochloryl,Chlorophenothane,TbisC-ethane,p',p'-DDT,4,4' DDT,4,4' Dichlorodiphenyltrichloroethane,TbisC ethane
D001140	Aroclors	Industrial chemicals which have become widespread environmental pollutants. Each aroclor is a mixture of chlorinated biphenyls (1200 series) or chlorinated terphenyls (5400 series) or a combination of both (4400 series).	Aroclor