BIOMAT Database Logo BIOMAT Database Logo

Concentration and molecular weight distribution of hyaluronate in synovial fluid from clinically normal horses and horses with diseased joints. 1994

R M Tulamo, and T Heiskanen, and M Salonen
Department of Clinical Sciences, College of Veterinary Medicine, Helsinki, Finland.

High molecular weight (MW) hyaluronate (HA) is an integral part of synovial fluid (SF), regulating many important physiologic and pathophysiologic mechanisms. Many of its effects depend on, or are reflected in, the concentration and MW of HA. High-performance liquid chromatography was used to assess simultaneously the concentration and MW of HA in SF obtained from horses with various arthritides: acute traumatic arthritis; chronic traumatic arthritis, including degenerative joint disease (DJD); and infectious arthritis. The size-exclusion column was calibrated, using appropriate HA concentration and MW standards, before the high-performance liquid chromatographic assays of the SF samples. Calibration of the column disclosed that the maximal limit for MW estimation of HA was around 3 million. In control joints, MW of HA ranged from 2 to 3 x 10(6) (mean 2.5 x 10(6)) and did not differ significantly from MW of HA in SF from horses with acute or chronic traumatic arthritis (mean 2 x 10(6); range 1.5 to 3 x 10(6)). Interestingly, a small amount of HA of moderately high MW (approx 1 to 1.5 x 10(6)) was detected in chromatograms of SF from infected joints. This degree of polymerization of SF HA was significantly (P < 0.01) lower, compared with that for control joints. There was no difference in mean (+/- SD) concentration of HA between control joints and joints with acute or chronic traumatic arthritis (0.33 +/- 0.12 g/L vs 0.18 +/- 0.03 g/L or 0.23 +/- 0.12 g/L), indicating that SF HA concentration probably should not be used as a diagnostic marker for the condition.(ABSTRACT TRUNCATED AT 250 WORDS)

UI MeSH Term Description Entries
D007592 Joint Diseases Diseases involving the JOINTS. Arthropathies,Arthropathy,Joint Disease
D007794 Lameness, Animal A departure from the normal gait in animals. Gait Disorders, Animal,Animal Gait Disorder,Animal Gait Disorders,Animal Lameness,Animal Lamenesses,Disorder, Animal Gait,Disorders, Animal Gait,Gait Disorder, Animal,Lamenesses, Animal
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D012016 Reference Values The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality. Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D002908 Chronic Disease Diseases which have one or more of the following characteristics: they are permanent, leave residual disability, are caused by nonreversible pathological alteration, require special training of the patient for rehabilitation, or may be expected to require a long period of supervision, observation, or care (Dictionary of Health Services Management, 2d ed). For epidemiological studies chronic disease often includes HEART DISEASES; STROKE; CANCER; and diabetes (DIABETES MELLITUS, TYPE 2). Chronic Condition,Chronic Illness,Chronically Ill,Chronic Conditions,Chronic Diseases,Chronic Illnesses,Condition, Chronic,Disease, Chronic,Illness, Chronic
D006734 Horse Diseases Diseases of domestic and wild horses of the species Equus caballus. Equine Diseases,Disease, Equine,Disease, Horse,Diseases, Equine,Diseases, Horse,Equine Disease,Horse Disease
D006736 Horses Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest. Equus caballus,Equus przewalskii,Horse, Domestic,Domestic Horse,Domestic Horses,Horse,Horses, Domestic
D006820 Hyaluronic Acid A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA. Amo Vitrax,Amvisc,Biolon,Etamucine,Healon,Hyaluronan,Hyaluronate Sodium,Hyvisc,Luronit,Sodium Hyaluronate,Acid, Hyaluronic,Hyaluronate, Sodium,Vitrax, Amo
D000208 Acute Disease Disease having a short and relatively severe course. Acute Diseases,Disease, Acute,Diseases, Acute

Related Publications

R M Tulamo, and T Heiskanen, and M Salonen
Hyaluronate and large molecular weight proteoglycans in synovial fluid from horses with various arthritides.
June 1996, American journal of veterinary research,
R M Tulamo, and T Heiskanen, and M Salonen
Hyaluronate concentration in tracheal lavage fluid from clinically normal horses and horses with chronic obstructive pulmonary disease.
July 1997, American journal of veterinary research,
R M Tulamo, and T Heiskanen, and M Salonen
Hyaluronic acid concentration in synovial fluid from normal and arthritic joints of horses.
January 1984, Australian veterinary journal,
R M Tulamo, and T Heiskanen, and M Salonen
Concentration and molecular weight of sodium hyaluronate in synovial fluid from patients with rheumatoid arthritis and other arthropathies.
December 1985, Annals of the rheumatic diseases,
R M Tulamo, and T Heiskanen, and M Salonen
Cortisol in synovial fluid from normal and diseased canine joints--a preliminary report.
November 1974, The Journal of small animal practice,
R M Tulamo, and T Heiskanen, and M Salonen
Lactic acid concentration in peritoneal fluid of normal and diseased horses.
July 1977, Research in veterinary science,
R M Tulamo, and T Heiskanen, and M Salonen
Concentrations of keratan sulfate in plasma and synovial fluid from clinically normal horses and horses with joint disease.
February 1997, Journal of the American Veterinary Medical Association,
R M Tulamo, and T Heiskanen, and M Salonen
Hyaluronate in normal human synovial fluid.
January 1958, The Journal of clinical investigation,
R M Tulamo, and T Heiskanen, and M Salonen
Synovial fluid cell counts and total protein concentration in clinically normal fetlock joints of young dromedarian camels.
June 2006, Journal of veterinary medicine. A, Physiology, pathology, clinical medicine,
R M Tulamo, and T Heiskanen, and M Salonen
Effects of intraarticular corticosteroid and sodium hyaluronate injections on synovial fluid production and synovial fluid content of sodium hyaluronate and proteoglycans in normal equine joints.
February 1993, Zentralblatt fur Veterinarmedizin. Reihe A,
Copied contents to your clipboard!