BIOMAT Database Logo BIOMAT Database Logo

Comparison of the SmaI-digested chromosomes of Staphylococcus epidermidis and the closely related species Staphylococcus capitis and Staphylococcus caprae. 1994

C G George, and W E Kloos
Department of Genetics, North Carolina University, Raleigh 27695-7614.

Pulsed-field gel electrophoresis was used to examine the chromosomal polymorphisms existing within and between four closely related members of the Staphylococcus epidermidis species group, S. epidermidis, Staphylococcus caprae, Staphylococcus capitis subsp. capitis, and S. capitis subsp. ureolyticus. SmaI was chosen as the restriction endonuclease for this study because it generated only a few well-separated chromosomal fragments. Each of the species and subspecies showed distinct SmaI digest patterns. The strains examined in this study were collected over a 20-year period from various geographical locations. The results indicate that DNA fragment patterns are unique to each species and subspecies and represent a reasonably stable component in the chromosome structure. S. caprae and S. capitis demonstrated considerable conservation in chromosome structure as indicated by the large numbers of conserved SmaI digest fragments. The polymorphisms found within each species appear to be linked to the species' character variability. The genome size of each Staphylococcus strain was extrapolated from the SmaI digest fragment pattern obtained by pulsed-field gel electrophoresis. The average genome size for S. epidermidis is 2,364 +/- 119 kb; for S. caprae strains from humans it is 2,600 +/- 157 kb and for S. caprae strains from goats it is 2,493 +/- 15 kb; for S. capitis subsp. capitis it is 2,456 +/- 71 kb; and for S. capitis subsp. ureolyticus it is 2,276 +/- 90 kb.

UI MeSH Term Description Entries
D010802 Phylogeny The relationships of groups of organisms as reflected by their genetic makeup. Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D011110 Polymorphism, Genetic The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level. Gene Polymorphism,Genetic Polymorphism,Polymorphism (Genetics),Genetic Polymorphisms,Gene Polymorphisms,Polymorphism, Gene,Polymorphisms (Genetics),Polymorphisms, Gene,Polymorphisms, Genetic
D002876 Chromosomes, Bacterial Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell. Bacterial Chromosome,Bacterial Chromosomes,Chromosome, Bacterial
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D006041 Goats Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP. Capra,Capras,Goat
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species
D013210 Staphylococcus A genus of gram-positive, facultatively anaerobic, coccoid bacteria. Its organisms occur singly, in pairs, and in tetrads and characteristically divide in more than one plane to form irregular clusters. Natural populations of Staphylococcus are found on the skin and mucous membranes of warm-blooded animals. Some species are opportunistic pathogens of humans and animals.
D013212 Staphylococcus epidermidis A species of STAPHYLOCOCCUS that is a spherical, non-motile, gram-positive, chemoorganotrophic, facultative anaerobe. Mainly found on the skin and mucous membrane of warm-blooded animals, it can be primary pathogen or secondary invader.

Related Publications

C G George, and W E Kloos
Complete genome sequencing of three human clinical isolates of Staphylococcus caprae reveals virulence factors similar to those of S. epidermidis and S. capitis.
November 2018, BMC genomics,
C G George, and W E Kloos
Occurrence of protein A in Staphylococcus aureus and closely related Staphylococcus species.
November 1979, Journal of clinical microbiology,
C G George, and W E Kloos
Comparison of identification systems for Staphylococcus epidermidis and other coagulase-negative Staphylococcus species.
March 1994, Diagnostic microbiology and infectious disease,
C G George, and W E Kloos
[Classification of Staphylococcus epidermidis species].
January 1971, Medycyna doswiadczalna i mikrobiologia,
C G George, and W E Kloos
Subclinical mastitis and antimicrobial susceptibility of Staphylococcus caprae and Staphylococcus epidermidis isolated from two Italian goat herds.
May 2005, Journal of dairy science,
C G George, and W E Kloos
Distribution of Staphylococcus species among human clinical specimens and emended description of Staphylococcus caprae.
July 1998, Journal of clinical microbiology,
C G George, and W E Kloos
Staphylococcus caprae: an emerging pathogen related to infective endocarditis.
September 2023, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases,
C G George, and W E Kloos
Comparative genomics of Staphylococcus capitis reveals species determinants.
January 2022, Frontiers in microbiology,
C G George, and W E Kloos
Mixed species biofilms of Candida albicans and Staphylococcus epidermidis.
April 2002, Journal of medical microbiology,
C G George, and W E Kloos
Comparison of epidemiologic markers for Staphylococcus epidermidis.
July 1986, Journal of clinical microbiology,
Copied contents to your clipboard!