Biomaterial Database

Two distinct NAD(P)H-dependent redox enzymes isolated from onion root plasma membranes. 1994

A Serrano, and J M Villalba, and J A González-Reyes, and P Navas, and F Córdoba

Departamento de Biología Celular, Universidad de Córdoba, Spain.

Plasma membranes purified by two-phase partition from onion roots catalyzed the NAD(P)H-dependent reduction of a variety of electron acceptor such as ferricyanide, quinones, dyes and ascorbate free radical. Among these, NAD(P)H-ferricyanide and -quinone oxidoreductase activities were effectively solubilized by Triton X-100. Both oxidoreductase activities were bound to an affinity column of Blue-Sepharose CL 6B. NADH eluted a redox enzyme showing more juglone than ferricyanide-dependent activity. Ulterior unspecific elution with salt allowed us to the partial purification of a different redox enzyme of about 31 kDa that reduced better ferricyanide than quinones and constituted the bulk of solubilized redox activity.

UI	MeSH Term	Description	Entries
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D009247	NADH, NADPH Oxidoreductases	A group of oxidoreductases that act on NADH or NADPH. In general, enzymes using NADH or NADPH to reduce a substrate are classified according to the reverse reaction, in which NAD+ or NADP+ is formally regarded as an acceptor. This subclass includes only those enzymes in which some other redox carrier is the acceptor. (Enzyme Nomenclature, 1992, p100) EC 1.6.	Oxidoreductases, NADH, NADPH,NADPH Oxidoreductases NADH,Oxidoreductases NADH, NADPH
D002462	Cell Membrane	The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.	Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D003579	Cytochrome Reductases		Reductases, Cytochrome
D004579	Electron Transport	The process by which ELECTRONS are transported from a reduced substrate to molecular OXYGEN. (From Bennington, Saunders Dictionary and Encyclopedia of Laboratory Medicine and Technology, 1984, p270)	Respiratory Chain,Chain, Respiratory,Chains, Respiratory,Respiratory Chains,Transport, Electron
D000490	Allium	A genus of the plant family Amaryllidaceae. Many produce pungent, often bacteriostatic and physiologically active compounds and are used as VEGETABLES; CONDIMENTS; and medicament, the latter in traditional medicine.	Alliaceae
D016660	NAD(P)H Dehydrogenase (Quinone)	A flavoprotein that reversibly catalyzes the oxidation of NADH or NADPH by various quinones and oxidation-reduction dyes. The enzyme is inhibited by dicoumarol, capsaicin, and caffeine.	DT Diaphorase,Menadione Reductase,Phylloquinone Reductase,Quinone Reductase,Vitamin K Reductase,Diaphorase, DT,Reductase, Menadione,Reductase, Phylloquinone,Reductase, Quinone,Reductase, Vitamin K
D042966	Cytochrome-B(5) Reductase	A FLAVOPROTEIN oxidoreductase that occurs both as a soluble enzyme and a membrane-bound enzyme due to ALTERNATIVE SPLICING of a single mRNA. The soluble form is present mainly in ERYTHROCYTES and is involved in the reduction of METHEMOGLOBIN. The membrane-bound form of the enzyme is found primarily in the ENDOPLASMIC RETICULUM and outer mitochondrial membrane, where it participates in the desaturation of FATTY ACIDS; CHOLESTEROL biosynthesis and drug metabolism. A deficiency in the enzyme can result in METHEMOGLOBINEMIA.	Methemoglobin Reductase,NADH Cytochrome B5 Reductase,NADH Methemoglobin Reductase,NADH-Cytochrome B5 Reductase,NADH-Ferrihemoglobin Reductase,B5 Reductase, NADH-Cytochrome,Methemoglobin Reductase, NADH,NADH Ferrihemoglobin Reductase,Reductase, Methemoglobin,Reductase, NADH Methemoglobin,Reductase, NADH-Ferrihemoglobin