-transparent.png){kind=logo}
A simple and sensitive colorimetric assay for serum diamine oxidase (DAO) activity was based on a coupled reaction with peroxidase and a new chromogen, 10-(carboxymethyl-aminocarbonyl)-3,7-bis(dimethylamino) phenothiazine sodium salt (DA-67). In the presence of peroxidase and DA-67, peroxidase catalyzes the formation of methylene blue having an absorption maximum at 668 nm. The proposed method eliminates the interferences occurring in serum with use of ascorbate oxidase and stops the reaction with sodium diethyldithiocarbamate, leaving the methylene blue in the reaction mixture stable for about 2 h. Low normal basal values of serum DAO can be determined in the range 2.8-9.0 units/l. Since all reagents are commercially available the method is suitable for the clinical laboratory.
K Takagi, and
M Nakao, and
Y Ogura, and
T Nabeshima, and
A Kunii
July 1987,
Chemical & pharmaceutical bulletin,
K Takagi, and
M Nakao, and
Y Ogura, and
T Nabeshima, and
A Kunii
March 1975,
Analytical biochemistry,
K Takagi, and
M Nakao, and
Y Ogura, and
T Nabeshima, and
A Kunii
October 1968,
The Journal of pharmacology and experimental therapeutics,
K Takagi, and
M Nakao, and
Y Ogura, and
T Nabeshima, and
A Kunii
February 1981,
Analytical biochemistry,
K Takagi, and
M Nakao, and
Y Ogura, and
T Nabeshima, and
A Kunii
January 1998,
Methods in molecular biology (Clifton, N.J.),
K Takagi, and
M Nakao, and
Y Ogura, and
T Nabeshima, and
A Kunii
July 1984,
Journal of chromatography,
K Takagi, and
M Nakao, and
Y Ogura, and
T Nabeshima, and
A Kunii
April 1989,
Clinical chemistry,
K Takagi, and
M Nakao, and
Y Ogura, and
T Nabeshima, and
A Kunii
July 1983,
Clinical chemistry,
K Takagi, and
M Nakao, and
Y Ogura, and
T Nabeshima, and
A Kunii
July 1974,
Acta obstetrica et gynaecologica Japonica,
K Takagi, and
M Nakao, and
Y Ogura, and
T Nabeshima, and
A Kunii
January 1986,
Biology of the neonate,
