In situ hybridization of luteinizing hormone/human chorionic gonadotropin receptor messenger ribonucleic acid during hormone-induced down-regulation and the subsequent recovery in rat corpus luteum. 1994

H Peegel, and J Randolph, and A R Midgley, and K M Menon
Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor 48109-0278.

Previous studies have shown that injection of a pharmacological dose of hCG to rats primed with PMSG/hCG results in a loss of hCG binding in the luteinized ovary, which is closely coupled with the loss of LH/hCG receptor (LH/hCG-R) messenger RNA (mRNA). The time course of down-regulation of the receptor mRNA reveals that mRNA totally disappears 24 h after the hormone injection, but fully recovers by 72 h. The purpose of this study was to determine by in situ hybridization whether the recovery of the receptor mRNA occurs in preexisting or newly formed corpora lutea. Twenty-one-day-old female rats were treated with 50 IU PMSG, followed 56 h later by a single injection of hCG. On day 5 after the hCG injection, one group of rats was treated with a desensitizing dose of hCG, and a control group received saline. The ovaries were collected 6, 12, 24, 48, 72, and 96 h after the treatments and were processed for in situ hybridization using 35S antisense RNA synthesized from a 750-mer LH/hCG-R complementary DNA. In control ovaries, heavily labeled LH/hCG-R mRNA-containing cells were observed in the numerous corpora lutea. Ribonuclease pretreatment of the sections eliminated the signal, and no specific hybridization was observed when sense strand probe was used. No hybridization to the granulosa cells was seen. Some hybridization occurred to the theca interna, but the intensity was lower than that in the corpora lutea. Time-course studies showed a marked decline in the hCG-R mRNA signals in corpora lutea as early as 6 h after hCG injection, with a maximum loss of receptor mRNA by 24 h. After 48 h, hCG-R mRNA reappeared in preexisting corpora lutea, with the intensity of the hybridization signal equaling that in corpora lutea of controls. New corpora lutea could not be identified at any time after injection of the down-regulating dose of hCG. As down-regulated receptor mRNA recovered in preexisting, not new, corpora lutea, hormone-induced loss of luteal cell receptors would appear to be reversible.

UI MeSH Term Description Entries
D007279 Injections, Subcutaneous Forceful administration under the skin of liquid medication, nutrient, or other fluid through a hollow needle piercing the skin. Subcutaneous Injections,Injection, Subcutaneous,Subcutaneous Injection
D010053 Ovary The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE. Ovaries
D011974 Receptors, LH Those protein complexes or molecular sites on the surfaces and cytoplasm of gonadal cells that bind luteinizing or chorionic gonadotropic hormones and thereby cause the gonadal cells to synthesize and secrete sex steroids. The hormone-receptor complex is internalized from the plasma membrane and initiates steroid synthesis. Chorionic Gonadotropin Receptors,Human Chorionic Gonadotropin Receptors,ICSH Receptors,LH Receptors,LH-hCG Receptor,LH-hCG Receptors,Luteinizing Hormone Receptors,Lutropin Receptor,Lutropin Receptors,Receptors, Chorionic Gonadotropin,Receptors, Human Chorionic Gonadotropin,Receptors, Interstitial Cell-Stimulating Hormone,Receptors, Luteinizing Hormone,hCG Receptors,Chorionic Gonadotropin Receptor,Human Chorionic Gonadotropin Receptor,LH Receptor,Luteinizing Hormone Receptor,Receptors, ICSH,Receptors, Interstitial Cell Stimulating Hormone,Receptors, LH-hCG,Receptors, Lutropin,Receptors, hCG,hCG Receptor,Gonadotropin Receptor, Chorionic,Gonadotropin Receptors, Chorionic,Hormone Receptor, Luteinizing,Hormone Receptors, Luteinizing,LH hCG Receptor,LH hCG Receptors,Receptor, Chorionic Gonadotropin,Receptor, LH,Receptor, LH-hCG,Receptor, Luteinizing Hormone,Receptor, Lutropin,Receptor, hCG,Receptors, LH hCG
D003338 Corpus Luteum The yellow body derived from the ruptured OVARIAN FOLLICLE after OVULATION. The process of corpus luteum formation, LUTEINIZATION, is regulated by LUTEINIZING HORMONE. Corpora Lutea,Lutea, Corpora
D005260 Female Females
D006063 Chorionic Gonadotropin A gonadotropic glycoprotein hormone produced primarily by the PLACENTA. Similar to the pituitary LUTEINIZING HORMONE in structure and function, chorionic gonadotropin is involved in maintaining the CORPUS LUTEUM during pregnancy. CG consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is virtually identical to the alpha subunits of the three pituitary glycoprotein hormones (TSH, LH, and FSH), but the beta subunit is unique and confers its biological specificity (CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN). Chorionic Gonadotropin, Human,HCG (Human Chorionic Gonadotropin),Biogonadil,Choriogonadotropin,Choriogonin,Chorulon,Gonabion,Human Chorionic Gonadotropin,Pregnyl,Gonadotropin, Chorionic,Gonadotropin, Human Chorionic
D006728 Hormones Chemical substances having a specific regulatory effect on the activity of a certain organ or organs. The term was originally applied to substances secreted by various ENDOCRINE GLANDS and transported in the bloodstream to the target organs. It is sometimes extended to include those substances that are not produced by the endocrine glands but that have similar effects. Hormone,Hormone Receptor Agonists,Agonists, Hormone Receptor,Receptor Agonists, Hormone
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor

Related Publications

H Peegel, and J Randolph, and A R Midgley, and K M Menon
July 1991, Endocrinology,
H Peegel, and J Randolph, and A R Midgley, and K M Menon
January 1996, Biology of reproduction,
H Peegel, and J Randolph, and A R Midgley, and K M Menon
March 1989, The Journal of clinical endocrinology and metabolism,
H Peegel, and J Randolph, and A R Midgley, and K M Menon
January 1994, Endocrinology,
H Peegel, and J Randolph, and A R Midgley, and K M Menon
February 1999, Fertility and sterility,
H Peegel, and J Randolph, and A R Midgley, and K M Menon
April 1995, The Journal of clinical endocrinology and metabolism,
Copied contents to your clipboard!