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The tendency of both labeled and unlabeled beta-amyloid to bind in solution to C1q, the recognition species in the complement cascade, was examined using both hydrodynamic and spectroscopic methods. Potential binding interactions were evaluated using a purified synthetic beta-amyloid 1-40 sequence, alone, and selectively labeled at the amino terminus with spectroscopic probes. The probes permitted both absorbance and fluorescence analyses of beta-amyloid binding interactions. Under conditions used for the analyses beta-amyloid exists exclusively as a monomer in solution, and C1q retains an intact quaternary structure and is capable of binding to IgM. When mixed together the monomeric beta-amyloid does not bind to, or interact with, the complement C1q at concentrations below approximately 100 microM. The data suggest that if beta-amyloid toxicity is associated with complement activation in Alzheimer's disease then monomeric beta-amyloid is likely not responsible for activation through the classical complement pathway.
S W Snyder, and
G T Wang, and
L Barrett, and
U S Ladror, and
D Casuto, and
C M Lee, and
G A Krafft, and
R B Holzman, and
T F Holzman
December 2023,
Proceedings of the National Academy of Sciences of the United States of America,
S W Snyder, and
G T Wang, and
L Barrett, and
U S Ladror, and
D Casuto, and
C M Lee, and
G A Krafft, and
R B Holzman, and
T F Holzman
January 1999,
Neurobiology of aging,
S W Snyder, and
G T Wang, and
L Barrett, and
U S Ladror, and
D Casuto, and
C M Lee, and
G A Krafft, and
R B Holzman, and
T F Holzman
July 2001,
The Journal of investigative dermatology,
S W Snyder, and
G T Wang, and
L Barrett, and
U S Ladror, and
D Casuto, and
C M Lee, and
G A Krafft, and
R B Holzman, and
T F Holzman
February 1990,
European journal of immunology,
S W Snyder, and
G T Wang, and
L Barrett, and
U S Ladror, and
D Casuto, and
C M Lee, and
G A Krafft, and
R B Holzman, and
T F Holzman
April 2003,
Journal of neuroimmunology,
S W Snyder, and
G T Wang, and
L Barrett, and
U S Ladror, and
D Casuto, and
C M Lee, and
G A Krafft, and
R B Holzman, and
T F Holzman
May 1997,
The American journal of pathology,
S W Snyder, and
G T Wang, and
L Barrett, and
U S Ladror, and
D Casuto, and
C M Lee, and
G A Krafft, and
R B Holzman, and
T F Holzman
January 2019,
Platelets,
S W Snyder, and
G T Wang, and
L Barrett, and
U S Ladror, and
D Casuto, and
C M Lee, and
G A Krafft, and
R B Holzman, and
T F Holzman
February 2008,
Journal of neurochemistry,
S W Snyder, and
G T Wang, and
L Barrett, and
U S Ladror, and
D Casuto, and
C M Lee, and
G A Krafft, and
R B Holzman, and
T F Holzman
August 1997,
Biochemical and biophysical research communications,
S W Snyder, and
G T Wang, and
L Barrett, and
U S Ladror, and
D Casuto, and
C M Lee, and
G A Krafft, and
R B Holzman, and
T F Holzman
May 2002,
Journal of immunology (Baltimore, Md. : 1950),
