Biomaterial Database

Superiority of amphotericin over nystatin in Thayer-Martin medium. 1975

E Whitbeck, and N Shemonsky, and M E Levison

Vaginal and cervical specimens taken for the detection of gonorrhea frequently contain Candida albicans. Thayer-Martin medium containing nystatin (VCTN) is unsatisfactory because nystatin fails to selectively inhibit C. albicans. Thayer-Martin medium containing no nystatin was prepared with varying concentrations of amphotericin (VCTA) added, and this latter medium was compared with VCTN for its ability to selectively inhibit varying numbers of C. albicans. The effect of amphotericin on the recovery of varying numbers of Neisseria gonorrhoeae was also studied. VCTN failed to inhibit 10(1) to 10(6)C. albicans. VCTA suppressed growth of up to 10(6)C. albicans. The optimal concentration of amphotericin B appeared to be 100 mug/ml. VCTA containing 100 mug of amphotericin B per ml (VCTA 100) was not inhibitory for N. gonorrhoeae when a dense population was applied with a replicator. However, it was slightly inhibitory when 10(1) to 10(2)N. gonorrhoeae were applied over the entire surface of an agar plate, although the colony count on VCTA 100 was more than 50% of the counts on chocolate agar and 75% of the counts on VCTN. These properties of VCTA 100, when stored at 4 C, were stable for up to 2 weeks.

UI	MeSH Term	Description	Entries
D009344	Neisseria gonorrhoeae	A species of gram-negative, aerobic bacteria primarily found in purulent venereal discharges. It is the causative agent of GONORRHEA.	Diplococcus gonorrhoeae,Gonococcus,Gonococcus neisseri,Merismopedia gonorrhoeae,Micrococcus der gonorrhoe,Micrococcus gonococcus,Micrococcus gonorrhoeae
D009761	Nystatin	Macrolide antifungal antibiotic complex produced by Streptomyces noursei, S. aureus, and other Streptomyces species. The biologically active components of the complex are nystatin A1, A2, and A3.	Fungicidin,Mycostatin,Nilstat,Nystatin A1,Nystatin A2,Nystatin A3,Nystatin G,Stamicin,Stamycin
D002176	Candida albicans	A unicellular budding fungus which is the principal pathogenic species causing CANDIDIASIS (moniliasis).	Candida albicans var. stellatoidea,Candida stellatoidea,Dematium albicans,Monilia albicans,Myceloblastanon albicans,Mycotorula albicans,Parasaccharomyces albicans,Procandida albicans,Procandida stellatoidea,Saccharomyces albicans,Syringospora albicans
D002584	Cervix Uteri	The neck portion of the UTERUS between the lower isthmus and the VAGINA forming the cervical canal.	Cervical Canal of the Uterus,Cervical Canal, Uterine,Ectocervix,Endocervical Canal,Endocervix,External Os Cervix,External Os of the Cervix,Uterine Cervical Canal,Cervix,Cervixes,Uterine Cervix,Canal, Endocervical,Canal, Uterine Cervical,Cervix, External Os,Cervix, Uterine,Endocervical Canals,Uterine Cervical Canals
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D005260	Female		Females
D000666	Amphotericin B	Macrolide antifungal antibiotic produced by Streptomyces nodosus obtained from soil of the Orinoco river region of Venezuela.	Amphocil,Amphotericin,Amphotericin B Cholesterol Dispersion,Amphotericin B Colloidal Dispersion,Fungizone
D014621	Vagina	The genital canal in the female, extending from the UTERUS to the VULVA. (Stedman, 25th ed)	Vaginas