Biomaterial Database

Regulation of Na and Cl transport in sheep distal airways. 1994

F J Al-Bazzaz

Department of Medicine, Veteran's Affairs Westside Medical Center, Chicago, Illinois.

To investigate ion transport function of distal airways mucosa we dissected and isolated segments of sheep bronchioles (outside diameter 664 +/- 10 microns, means +/- SE). Both ends of a segment were cannulated with glass micropipettes, and the preparation was placed in a bath and perfused with oxygenated Krebs-Henseleit solution at 37 degrees C. Luminal amiloride (0.1 mM) reduced potential difference (PD) from 3.06 +/- 0.68 mV to 1.08 +/- 0.24 mV (n = 5, P < 0.02). Submucosal ouabain (0.1 mM) decreased PD from 1.82 +/- 0.38 to 0.07 +/- 0.02 mV (n = 5, P < 0.009). Submucosal bumetanide (0.1 mM) caused a decline in PD from 3.44 +/- 0.98 to 2.75 +/- 0.71 mV (n = 19, P < 0.03). Exposure of the lumen to Cl channel blocker diphenylamine-2-carboxylate (1 mM) depolarized the bronchiole from a PD of 4.46 +/- 1.7 mV to 2.50 +/- 0.97 mV (n = 7, P < 0.03). Submucosal adenosine 3',5'-cyclic monophosphate (cAMP) analogue, 8-(4-chlorophenylthio)-cAMP (0.1 mM), raised PD from 3.45 +/- 1.08 to 3.82 +/- 1.24 mV (n = 10, P < 0.05). In eight experiments submucosal 0.1 mM carbachol, which elevates cytosolic Ca, resulted in rapid hyperpolarization (P < 0.02) followed by amiloride-inhibitable slow depolarization (P < 0.05). The data provide evidence for the presence in the sheep bronchiolar epithelium of active Na absorption that depends on a basolaterally located Na-K-ATPase ion pump. Increased cytosolic Ca probably inhibits Na absorption. The epithelium also has a Cl secretory process through apical Cl channels.

UI	MeSH Term	Description	Entries
D010042	Ouabain	A cardioactive glycoside consisting of rhamnose and ouabagenin, obtained from the seeds of Strophanthus gratus and other plants of the Apocynaceae; used like DIGITALIS. It is commonly used in cell biological studies as an inhibitor of the NA(+)-K(+)-EXCHANGING ATPASE.	Acocantherin,G-Strophanthin,Acolongifloroside K,G Strophanthin
D001980	Bronchi	The larger air passages of the lungs arising from the terminal bifurcation of the TRACHEA. They include the largest two primary bronchi which branch out into secondary bronchi, and tertiary bronchi which extend into BRONCHIOLES and PULMONARY ALVEOLI.	Primary Bronchi,Primary Bronchus,Secondary Bronchi,Secondary Bronchus,Tertiary Bronchi,Tertiary Bronchus,Bronchi, Primary,Bronchi, Secondary,Bronchi, Tertiary,Bronchus,Bronchus, Primary,Bronchus, Secondary,Bronchus, Tertiary
D002217	Carbachol	A slowly hydrolyzed CHOLINERGIC AGONIST that acts at both MUSCARINIC RECEPTORS and NICOTINIC RECEPTORS.	Carbamylcholine,Carbacholine,Carbamann,Carbamoylcholine,Carbastat,Carbocholine,Carboptic,Doryl,Isopto Carbachol,Jestryl,Miostat,Carbachol, Isopto
D002712	Chlorides	Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.	Chloride,Chloride Ion Level,Ion Level, Chloride,Level, Chloride Ion
D004594	Electrophysiology	The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D005947	Glucose	A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.	Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D000242	Cyclic AMP	An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.	Adenosine Cyclic 3',5'-Monophosphate,Adenosine Cyclic 3,5 Monophosphate,Adenosine Cyclic Monophosphate,Adenosine Cyclic-3',5'-Monophosphate,Cyclic AMP, (R)-Isomer,Cyclic AMP, Disodium Salt,Cyclic AMP, Monoammonium Salt,Cyclic AMP, Monopotassium Salt,Cyclic AMP, Monosodium Salt,Cyclic AMP, Sodium Salt,3',5'-Monophosphate, Adenosine Cyclic,AMP, Cyclic,Adenosine Cyclic 3',5' Monophosphate,Cyclic 3',5'-Monophosphate, Adenosine,Cyclic Monophosphate, Adenosine,Cyclic-3',5'-Monophosphate, Adenosine,Monophosphate, Adenosine Cyclic
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001692	Biological Transport	The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.	Transport, Biological,Biologic Transport,Transport, Biologic
D012756	Sheep	Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.	Ovis,Sheep, Dall,Dall Sheep,Ovis dalli