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The effect of nickel from soluble NiCl2 on Cu-Zn superoxide dismutase (SOD) activity, as well as on rate of nitro blue tetrazolium reduction, was studied in vitro since lipid peroxidation has been implicated in cell damage by nickel insoluble compounds, whose toxicity and carcinogenicity are well established. The physical and chemical nature of nickel compounds is one of the key determinations of its toxicity. Soluble nickel freely enter cells, but is just as readily excreted reducing the opportunity for production of lipid damage. Nickel from NiCl2 strongly activated SOD activity. In vitro addition of nickel chloride to a crude lung preparation altered the KM for SOD without changing the Vmax. Nickel chloride produced increased enzyme affinity to the substrate, because decreased (O2-) concentration that yields half-maximal velocity. The combination of nickel and SOD may contribute to stabilization of the particular conformation of SOD responsible for maximal catalytically activity.
E L Novelli, and
N L Rodrigues, and
B O Ribas
August 1992,
Transplantation proceedings,
E L Novelli, and
N L Rodrigues, and
B O Ribas
March 1991,
Biological trace element research,
E L Novelli, and
N L Rodrigues, and
B O Ribas
November 1985,
Annals of surgery,
E L Novelli, and
N L Rodrigues, and
B O Ribas
September 1991,
Journal of trace elements and electrolytes in health and disease,
E L Novelli, and
N L Rodrigues, and
B O Ribas
January 1981,
Bulletin europeen de physiopathologie respiratoire,
E L Novelli, and
N L Rodrigues, and
B O Ribas
July 1985,
Sheng li ke xue jin zhan [Progress in physiology],
E L Novelli, and
N L Rodrigues, and
B O Ribas
January 1996,
Advances in neurology,
E L Novelli, and
N L Rodrigues, and
B O Ribas
January 1984,
Methods in enzymology,
E L Novelli, and
N L Rodrigues, and
B O Ribas
May 1987,
Surgery,
E L Novelli, and
N L Rodrigues, and
B O Ribas
February 1992,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology,
