Biomaterial Database

Enhancement of baculovirus plaque assay in insect cell monolayers by DEAE-dextran. 1994

A L Carrascosa

Centro de Biología Molecular (C.S.I.C.-U.A.M.), Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.

The presence of DEAE-dextran in the agarose overlay, when titrating wild-type or recombinant baculoviruses by plaque assay, resulted in a higher definition and contrast of viral plaques and increased the plaque number and mean diameter at least by a factor of 2x on day 5 post infection. This increase was related to neither a larger production of infectious virus nor of recombinant protein and did not occur when the polycation was only present in the virus inoculum or when insect cell monolayers were preincubated for 60 min with it. The extension of the observations to a number of different recombinant viruses from varied sources, including several baculoviruses that could not consistently produce plaques in the absence of the polycation, substantiates the use of DEAE-dextran to perform a more reliable, faster and reproducible plaque assay of recombinant baculoviruses.

UI	MeSH Term	Description	Entries
D009036	Moths	Insects of the suborder Heterocera of the order LEPIDOPTERA.	Antheraea,Giant Silkmoths,Giant Silkworms,Silkmoths, Giant,Silkworms, Giant,Antheraeas,Giant Silkmoth,Giant Silkworm,Moth,Silkmoth, Giant,Silkworm, Giant
D010948	Viral Plaque Assay	Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.	Bacteriophage Plaque Assay,Assay, Bacteriophage Plaque,Assay, Viral Plaque,Assays, Bacteriophage Plaque,Assays, Viral Plaque,Bacteriophage Plaque Assays,Plaque Assay, Bacteriophage,Plaque Assay, Viral,Plaque Assays, Bacteriophage,Plaque Assays, Viral,Viral Plaque Assays
D011994	Recombinant Proteins	Proteins prepared by recombinant DNA technology.	Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D003637	DEAE-Dextran	Used as a support for ion-exchange chromatography.	DEAE Sephadex,Diethylaminoethyldextran,Pulsar,DEAE Dextran,Sephadex, DEAE
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D016367	Baculoviridae	Family of INSECT VIRUSES which contain polyhedron-shaped or ovocylindrical occlusion bodies. The genera include ALPHABACULOVIRUS; GAMMABACULOVIRUS; and DELTABACULOVIRUS (commonly known as NUCLEOPOLYHEDROVIRUSES) and BETABACULOVIRUS (commonly known as GRANULOVIRUSES). Baculovirus vectors are used for expression of foreign genes in insects and as BIOPESTICIDES for controlling insect populations.	Baculovirus,Baculoviruses