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Each of four common homozygous bovine serum transferrins Tf A, Tf D1, Tf D2, and Tf E gave only two main bands in polyacrylamide gel isoelectric focusing (PAGIEF). This is considered to be due to the co-migration of the main components 2a and 3a with the main components 2b and 3b, respectively, in PAGIEF. Ten phenotypes, which are controlled by four alleles TfA, TfD1, TfD2, and TfE, were distinguishable from each other in PAGIEF.
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