-transparent.png){kind=logo}
The lipopolysaccharide (LPS) of Haemophilus influenzae expresses a number of core oligosaccharide epitopes on its outer surface. The expression of individual epitopes is subject to frequent (approximately 1% bacteria/generation) reversible phase variation, as determined by colony immunoblots. We have used a microtechnique for the extraction of LPS from individual colonies, whose LPS antigenic phenotype has been identified, so that the LPS can be studied by tricine sodium dodecylsulphate polyacrylamide gel electrophoresis (T-SDS-PAGE). This avoids the introduction of heterogeneous phase-varying LPS which is inevitable if bacteria from colonies are grown in broth culture prior to LPS extraction and analysis. Using these techniques we have investigated the repertoire of LPS phase variation exhibited by H. influenzae strain RM7004 (a serotype b meningitis isolate). This technique will facilitate the study of bacteria in which there is variable LPS expression.
R J Roche, and
N J High, and
E R Moxon
January 1991,
Research in microbiology,
R J Roche, and
N J High, and
E R Moxon
June 1990,
Journal of bacteriology,
R J Roche, and
N J High, and
E R Moxon
December 1978,
The Journal of infectious diseases,
R J Roche, and
N J High, and
E R Moxon
August 1999,
Biochemical Society transactions,
R J Roche, and
N J High, and
E R Moxon
September 1983,
The Journal of infectious diseases,
R J Roche, and
N J High, and
E R Moxon
November 1998,
Molecular microbiology,
R J Roche, and
N J High, and
E R Moxon
June 1992,
The Journal of infectious diseases,
R J Roche, and
N J High, and
E R Moxon
April 2001,
European journal of biochemistry,
R J Roche, and
N J High, and
E R Moxon
October 1990,
Infection and immunity,
R J Roche, and
N J High, and
E R Moxon
August 1991,
Biochemical Society transactions,
