Biomaterial Database

Effect of the tyrosine kinase inhibitor tyrphostin on insulin binding and insulin imprinting of Tetrahymena. 1994

P Kovács, and G Csaba

Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.

After 10 min treatment with the tyrosine kinase inhibitor tyrphostin the fluorescein isothiocyanate (FITC)-insulin binding of Tetrahymena was increased. This binding characteristic was not changed when tyrphostin and insulin acted together. Consecutive treatments with 10 min tyrphostin and 10 min insulin further increased the FITC-insulin binding. Due to the effect of treatment with tyrphostin there was a decreased insulin binding in the offspring generations after 24 h. There was increased insulin binding in those cells of which the progenitor was treated not only by tyrphostin but also with insulin, but the insulin binding was still below the control level in this group. The increase of binding in the offspring generations of cells which were treated consecutively by tyrphostin and insulin compared with cells treated with only tyrphostin was not less than the increase of binding in the offspring of the untreated and insulin pretreated (24 h before) cells. This meant that tyrphostin had the potency to decrease insulin binding in the offspring generations but the development of imprinting was not influenced.

UI	MeSH Term	Description	Entries
D007328	Insulin	A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).	Iletin,Insulin A Chain,Insulin B Chain,Insulin, Regular,Novolin,Sodium Insulin,Soluble Insulin,Chain, Insulin B,Insulin, Sodium,Insulin, Soluble,Regular Insulin
D009570	Nitriles	Organic compounds containing the -CN radical. The concept is distinguished from CYANIDES, which denotes inorganic salts of HYDROGEN CYANIDE.	Nitrile
D011505	Protein-Tyrosine Kinases	Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors.	Tyrosine Protein Kinase,Tyrosine-Specific Protein Kinase,Protein-Tyrosine Kinase,Tyrosine Kinase,Tyrosine Protein Kinases,Tyrosine-Specific Protein Kinases,Tyrosylprotein Kinase,Kinase, Protein-Tyrosine,Kinase, Tyrosine,Kinase, Tyrosine Protein,Kinase, Tyrosine-Specific Protein,Kinase, Tyrosylprotein,Kinases, Protein-Tyrosine,Kinases, Tyrosine Protein,Kinases, Tyrosine-Specific Protein,Protein Kinase, Tyrosine-Specific,Protein Kinases, Tyrosine,Protein Kinases, Tyrosine-Specific,Protein Tyrosine Kinase,Protein Tyrosine Kinases,Tyrosine Specific Protein Kinase,Tyrosine Specific Protein Kinases
D002396	Catechols	A group of 1,2-benzenediols that contain the general formula R-C6H5O2.	Pyrocatechols,o-Dihydroxybenzenes,ortho-Dihydroxybenzenes,o Dihydroxybenzenes,ortho Dihydroxybenzenes
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013768	Tetrahymena	A genus of ciliate protozoa commonly used in genetic, cytological, and other research.	Tetrahymenas
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor
D020032	Tyrphostins	A family of synthetic protein tyrosine kinase inhibitors. They selectively inhibit receptor autophosphorylation and are used to study receptor function.	Tyrphostin