BIOMAT Database Logo BIOMAT Database Logo

Granulocyte activation and adhesion molecules during hemodialysis with cuprophane and a high-flux biocompatible membrane. 1994

C Combe, and M Pourtein, and V de Précigout, and A Baquey, and D Morel, and L Potaux, and P Vincendeau, and J H Bézian, and M Aparicio
Service d'Hémodialyse, Hôpital Pellegrin, Bordeaux, France.

Hemodialysis with complement-activating membranes, such as cuprophane, induces neutropenia and expression of the granulocyte adhesion receptor Mac-1 (CD11b/CD18), while hemodialysis with noncomplement-activating membranes does not. Increased expression of CD11b by neutrophils may mediate cuprophane-induced leukopenia. However, the rebound granulocytosis that follows leukopenia is not fully understood. Ten patients on regular hemodialysis were included in a cross-over study. Hemodialysis was performed for 2 weeks with cuprophane and 2 weeks with polyamide, a high-flux noncomplement-activating membrane. At the end of each period, the following parameters were determined during a hemodialysis session: C5a concentration by enzyme immunoassay and the neutrophil expression of CD11b, LFA-1 (CD11a/CD18), and the antigen recognized by MoF11 (MoF11 Ag), a monoclonal antibody that recognizes activated neutrophils, by immunofluorescence flow cytometry. Hemodialysis with cuprophane induced an increase in C5a concentration and in the expression of CD11b and MoF11 Ag, which were maximal after 15 minutes of hemodialysis, at the nadir of neutropenia. CD11b expression was maintained throughout hemodialysis, despite the reversal of neutropenia. Conversely, after peak expression, C5a and MoF11 Ag decreased as the neutrophil count increased to baseline values. Polyamide hemodialysis did not induce variations in C5a concentration, nor in CD11b and MoF11 Ag expression. CD11a/CD18 expression remained stable during hemodialysis with both membrane types. Neutrophil activation, as determined by MoF11 Ag expression, was correlated with the evolution of neutrophil count and C5a concentration during cuprophane hemodialysis, while CD11b expression was not correlated with neutrophil count throughout dialysis. A decrease in neutrophil activation could explain in part the detachment of neutrophils previously bound to endothelium and, therefore, the reversal of neutropenia.(ABSTRACT TRUNCATED AT 250 WORDS)

UI MeSH Term Description Entries
D008297 Male Males
D008567 Membranes, Artificial Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION. Artificial Membranes,Artificial Membrane,Membrane, Artificial
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D002482 Cellulose A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations. Alphacel,Avicel,Heweten,Polyanhydroglucuronic Acid,Rayophane,Sulfite Cellulose,alpha-Cellulose,Acid, Polyanhydroglucuronic,alpha Cellulose
D005260 Female Females
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D005455 Fluorescent Antibody Technique Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy. Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D006098 Granulocytes Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS. Granulocyte
D006435 Renal Dialysis Therapy for the insufficient cleansing of the BLOOD by the kidneys based on dialysis and including hemodialysis, PERITONEAL DIALYSIS, and HEMODIAFILTRATION. Dialysis, Extracorporeal,Dialysis, Renal,Extracorporeal Dialysis,Hemodialysis,Dialyses, Extracorporeal,Dialyses, Renal,Extracorporeal Dialyses,Hemodialyses,Renal Dialyses
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

C Combe, and M Pourtein, and V de Précigout, and A Baquey, and D Morel, and L Potaux, and P Vincendeau, and J H Bézian, and M Aparicio
Patient reactions and granulocyte degranulation during hemodialysis with cuprophane and polycarbonate membranes. A double-blind study.
January 1986, Blood purification,
C Combe, and M Pourtein, and V de Précigout, and A Baquey, and D Morel, and L Potaux, and P Vincendeau, and J H Bézian, and M Aparicio
Granulocyte activation during hemodialysis.
January 1986, Clinical nephrology,
C Combe, and M Pourtein, and V de Précigout, and A Baquey, and D Morel, and L Potaux, and P Vincendeau, and J H Bézian, and M Aparicio
[Anaphylactic reactions during hemodialysis: responsibility of the dialysis membrane (cuprophane)].
January 1984, Nephrologie,
C Combe, and M Pourtein, and V de Précigout, and A Baquey, and D Morel, and L Potaux, and P Vincendeau, and J H Bézian, and M Aparicio
Monocyte expression of adhesion molecules during low- and high-flux polysulfone hemodialysis and the effect of atorvastatin administration.
January 2010, Blood purification,
C Combe, and M Pourtein, and V de Précigout, and A Baquey, and D Morel, and L Potaux, and P Vincendeau, and J H Bézian, and M Aparicio
Hemodialysis with cuprophane membrane modulates interleukin-2 receptor expression.
May 1991, Kidney international,
C Combe, and M Pourtein, and V de Précigout, and A Baquey, and D Morel, and L Potaux, and P Vincendeau, and J H Bézian, and M Aparicio
[Changes in leukocyte counts using a cellulose acetate membrane (FB-T) during hemodialysis. A comparison with a cuprophane membrane].
September 1986, Hinyokika kiyo. Acta urologica Japonica,
C Combe, and M Pourtein, and V de Précigout, and A Baquey, and D Morel, and L Potaux, and P Vincendeau, and J H Bézian, and M Aparicio
Expression of adhesion molecules and activation markers on lymphocytes and monocytes during hemodialysis.
January 1997, Blood purification,
C Combe, and M Pourtein, and V de Précigout, and A Baquey, and D Morel, and L Potaux, and P Vincendeau, and J H Bézian, and M Aparicio
Effect of dialyzer geometry during hemodialysis with cuprophane membranes.
August 1992, Kidney international,
C Combe, and M Pourtein, and V de Précigout, and A Baquey, and D Morel, and L Potaux, and P Vincendeau, and J H Bézian, and M Aparicio
[Anaphylaxis during cuprophane hemodialysis--a report of 3 cases].
October 1985, Taiwan yi xue hui za zhi. Journal of the Formosan Medical Association,
C Combe, and M Pourtein, and V de Précigout, and A Baquey, and D Morel, and L Potaux, and P Vincendeau, and J H Bézian, and M Aparicio
Intraocular pressures during high-flux hemodialysis.
January 1990, Renal failure,
Copied contents to your clipboard!