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To localize functional loci on cytological maps of polytene chromosomes we propose to use 10-100 times stretched chromosomes. Three different ways of stretchening are briefly considered: the squash tissue preparation, when chromosomes are stretched by hydrodynamical forces; the treatment of isolated polytene chromosomes in 10-minus 4M EDTA OR 0.8M NaCL with subsequent change of these solution for saline when abrupt structural changes occur in chromosomes and they become morphologically homogeneous threads (Gruzdev and Belaya, 1973); and, finally, the use of microneedles of the micromanipulator. After an intense (ca. 100 times) stretchening, the autoradiography is sufficient to localize the loci within one micron length of double helical DNA molecule.
A D Gruzdev, and
G A Zaĭniev, and
N A Reznik
January 1978,
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A D Gruzdev, and
G A Zaĭniev, and
N A Reznik
November 1975,
Tsitologiia,
A D Gruzdev, and
G A Zaĭniev, and
N A Reznik
October 2010,
Genetika,
A D Gruzdev, and
G A Zaĭniev, and
N A Reznik
January 1999,
Advances in genetics,
A D Gruzdev, and
G A Zaĭniev, and
N A Reznik
January 1991,
Genetica,
A D Gruzdev, and
G A Zaĭniev, and
N A Reznik
April 2018,
Current genomics,
A D Gruzdev, and
G A Zaĭniev, and
N A Reznik
April 1978,
Biophysics of structure and mechanism,
A D Gruzdev, and
G A Zaĭniev, and
N A Reznik
January 1974,
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik,
A D Gruzdev, and
G A Zaĭniev, and
N A Reznik
January 2011,
PloS one,
A D Gruzdev, and
G A Zaĭniev, and
N A Reznik
January 1998,
Molekuliarnaia biologiia,
