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Radial immunodiffusion was used to determine the concentration of the third component of complement (C3) in the serum of 51 adult dogs with leishmaniasis and, for comparative purposes, in a control group of 30 healthy dogs. Animals in both groups were of different age, sex and breed. The mean concentration in healthy dogs was 93.3 +/- 6.6 (mean +/- SD), and in sick dogs 131.4 +/- 7.1, both expressed as a percentage of a reference standard. Statistically significant differences (P < 0.0004) were found between serum C3 concentrations in leishmania-infected and control groups, but no significant difference was recorded between males and females in either group.
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